Effect of n-1-naphthylpthalamic acid and 6-benzylaminopurine on root formation and the induction of new shoots in Agave spp.

Bebeto Garcia Rojas, Amaury Martin Arzate Fernandez, Jesús Ignacio Reyes Díaz, Tomás Héctor Norman Mondragón

Abstract


Background. The Agave genus is of great importance due to its ancestral use as a source of various products, which highlights the need to ensure its propagation. To achieve this, it is essential to understand the development of apical meristems, key structures in the formation of plant architecture. Thus, 6-Benzylaminopurine (6-BAP) acts as an endogenous cytokinin, stimulating buds and lateral roots, while n-1-naphthylphthalamic acid (NPA) functions as an auxin inhibitor affecting its transport in different organs. This knowledge is crucial to understand how these plant growth regulators influence the development of agave plants. Objective. To evaluate the effect of n-1-naphthylphthalamic acid and 6-benzylaminopurine on root formation and the induction of new shoots in Agave salmiana and A. angustifolia. Methodology. Seven treatments with different concentrations of NPA and 6-BAP were applied to two Agave species in acclimatization chambers and semi-hydroponic systems. Results. The results showed that Agave species responded variably in terms of root development. Furthermore, both NPA and 6-BAP, applied individually and in combination, were able to suppress apical dominance and stimulate the sprouting of new lateral buds. Implications. The findings of this research suggest the potential of these treatments as a method to improve multiplication rates and also provide an example of the interaction between auxins and cytokinins in the maintenance of meristems. Conclusions. The effect of NPA and 6-BAP treatments on root development was highly variable, while they were highly effective in the formation of new shoots.

Keywords


n-1-naphthylphthalamic acid; 6-benzylaminopurine; shoot apical meristem; root apical meristem; Agave.

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URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v28i3.60491

DOI: http://dx.doi.org/10.56369/tsaes.6049

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Copyright (c) 2025 Amaury-M. Arzate Fernandez, Bebeto Garcia Rojas, Jesús Ignacio Reyes Díaz, Tomás Héctor Norman Mondragón

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