Estimation of nuclear DNA content in germoplasm of Bouteloua curtipendula (Michx.) Torr. using flow cytometry

Edgar Eduardo Pozos Méndez, Evert Villanueva Sánchez, Martha Hernández Rodríguez, Adrián Raymundo Quero Carrillo, Hugo Alejandro Álvarez Hernández

Abstract


Background. Bouteloua curtipendula (Michx.) Torr. is a grass native to Mexico with a high capacity for adaptation in areas with limited moisture. It has physiological and morphological characteristics that are of interest in genetic improvement programmes for the restoration of degraded areas and the establishment of pastures for livestock grazing. In addition, flow cytometry can provide useful information in the study of the plant genome of this grass, such as nuclear DNA content (2C value) and its correlation with ploidy levels. Objective. To determine the nuclear DNA content of 57 ecotypes of Bouteloua curtipendula (Michx.) to infer their ploidy level. Methodology. Nuclear DNA content was estimated using flow cytometry. The internal standard was Zea mays L. The analysis considered average fluorescence intensity indices with a coefficient of variation <5%. Based on the fluorescence index and the 2C DNA value of the internal standard, the absolute DNA content (2C) in pg of each ecotype was obtained. The level of ploidy was inferred from the data obtained. Results. The range of nuclear DNA content of the ecotypes analysed ranged from 1.66 to 5.67 pg. Four different ploidy groups were found, with the tetraploid level predominating with 39 ecotypes. Implications and Conclusion. There is considerable variation in the nuclear DNA content of B. curtipendula (Michx.) Torr., a valuable forage grass for rainfed pastures, and flow cytometry was useful in determining this variation.

Keywords


Bouteloua curtipendula; DNA; ploidy; chromosomes

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References


Álvarez-Holguín, A., Morales-Nieto, C.R., Corrales-Lerma, R., Prieto-Amparán, J.A., Iracheta-Lara, I.Z., and Hernández-Quiroz, N.S. 2022. Estructura genética y aptitud ambiental de poblaciones de pasto banderita [Bouteloua curtipendula (Michx.) Torr.] en Chihuahua, México. Revista Mexicana De Ciencias Pecuarias, 13(3), pp. 830–845. https://doi.org/10.22319/rmcp.v13i3.5730

Arumuganathan, K. and Earle, E.D. 1991. Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter, 9(3), pp. 208-218. https://doi.org/10.1007/BF02672069

Arumuganathan, K., Tallury, S.P., Fraser, M.L., Bruneau, A.H., Qu, R. 1999. Nuclear DNA content of thirteen turfgrass species by flow cytometry. Crop Science, 39, pp. 1518-1521. https://doi.org/10.2135/cropsci1999.3951518x

Bennett, M.D. 1987. Variation in genomic form in plants and its ecological implications. New Phytologist, 06, pp. 177-200.

Bonos, S.A., Plumley, K.A. and Meyer, W.A. 2002. Ploidy Determination in Using Flow Cytometry and Morpgological Traits. Crop Science, 42(1), pp. 192. https://doi.org/10.2135/cropsci2002.1920

Chen, J., Xia, N., Wang, X., Beeson, R.C. and Chen, J. 2017. Ploidy Level, Karyotype and DNA Content in the Genus Lonicera. HortScience, 52(12), pp. 1680-1686. https://doi.org/10.21273/HORTSCI12489-17

Corrales-Lerma, R., Morales-Nieto, C.R., Melgoza-Castillo, A., Sierra-Tristán, J.S., Ortega-Gutiérrez, J.Á. and Méndez-Zamora, G. 2016. Caracterización de variedades de pasto banderita [Bouteloua curtipendula (Michx.) Torr.] recomendadas para rehabilitación de pastizales. Revista Mexicana de Ciencias Pecuarias, 7(2), pp. 201-211.

Do Valle, C. and Savidan, Y.H. 1996. Genetics, cytogenetics and reproductive biology of Brachiaria. In: Miles, J.W., Maass, B.L., Valle, C.B., Kumble, V. (eds.). Brachiaria: Biology, agronomy, and improvement. Centro Internacional de Agricultura Tropical, CIAT, Cali, CO. pp. 147-163.

Dolezel, J., Greihuber, J., and Suda, J. 2007. Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols, 2(9), pp. 2233-2244. https://doi.org/10.1038/nprot.2007.310

Eaton, T.D., Curley, R.J., Williamson, C., Jung, G. 2004. Determination of the level of variation in polyploidy among Kentucky bluegrass cultivars by means of flow cytometry. Crop Science, 44, pp. 2168-2174.

García, V.A. 1990. Plant Cytogenetic Techniques and Procedures. Postgraduate College. Montecillo, Mexico. pp. 144

Galbraith, D.W., Harkins, K.R., Maddox, J.M., Ayres, N.M., Sharma, D.P. and Firoozabady, E. 1983. Rapid flow cytophotometric analysis of the cell cycle in intact plant tissues. Science, 220, pp. 1049–1051.

Gastelum, M.F.I., Cantú, A.C.M., Uvalle, S.J.I., Lozano, C.E.A., Serna, L.R. and González, S.F.N. 2020. Importancia del matorral desértico micrófilo para el venado cola blanca (Odocoileus virginianus Mearns, 1898) en Coahuila. Revista Mexicana de Ciencias Forestales, 11(62), pp. 136-156. https://doi.org/10.29298/rmcf.v11i62.747

Hopkins A.A., Taliaferro, C.M., Christian, C.D.1996. Chromosome number and nuclear DNA content of several switchgrass populations. Crop Science, 36, pp. 1192-1195.

Johnson, P.C., Riordan, T.P. and Arumuganathan, K. 1998. Ploidy level determinations in buffalograss clones and populations. Crop Science, 38, pp. 478-482. https://doi.org/10.2135/cropsci1998.0011183X003800020034x

Kapadia, Z.J. and Gould, F.W. 1964. Biosystematic studies in the Bouteloua curtipendula complex. III. Pollen size as related to chromosome numbers. American Journal of Botany, 51, pp. 166–172. https://doi.org/10.2307/2440101

Levin, D.A. and Funderburg, S.W., 1979: Genome size in angiosperms: temperate versus tropical species. American Naturalist, 114, pp. 784-795.

Loureiro, J.C.M. 2009. Flow cytometric approaches to study plant genomes. Revista Científica y Técnica de Ecología y Medio Ambiente, Ecosistemas, 18(2), pp. 103-108.

Martínez, S.J.A., Durán, P.N., Ruiz, C.J.A., González, E.D.R. and Mena, M.S. 2020. Áreas con aptitud ambiental para [Bouteloua curtipendula (Michx.) Torr.] en México por efecto del cambio climático. Revista Mexicana de Ciencias Pecuarias, 11(2), pp. 49-62. https://doi.org/10.22319/rmcp.v11s2.4693

Morales, N.C.R., Quero, C.A.R. and Avedaño, A.C.H. 2007. Caracterización de la diversidad nativa del zacate banderita [Bouteloua curtipendula (Michx.) Torr.] mediante su nivel de ploidía. Revista Técnica Pecuaria en México, 45(3), pp. 263-278. http://www.redalyc.org/articulo.oa?id=61345302

Morales, N.C.R, Avendaño, A.C.H., Melgoza, C.A., Martínez, S. M. and Jurado, G.P. 2015. Morphological and molecular characterisation of populations of early foxtail (Setaria macrostachya Kunth) in Chihuahua, Mexico. Revista Internacional Botánica Experimental, 84, pp. 190-200.

Morales, N.C.R., Avedaño, A.C.H, Melgoza, C.A., Gil, V.K.C., Quero, C.A., Jurado, G.P. and Martínez, SQM. 2016. Caracterización morfológica y molecular de poblaciones de pasto banderita (Boteloua curtipendula) en Chihuahua, México. Revista Mexicana de Ciencias Pecuarias, 7(4), pp. 455-469. http://www.scielo.org.mx/pdf/rmcp/v7n4/24486698-rmcp-7-04-00455.pdf

Nodal-Moreno, S.A., Palomino, G., Almaguer, S.P., Blanco, M.F., Barientos, L.L. and Flores G.J. 2019 Análisis del tamaño del genoma, poliploidía y patrón endopoliploide en poblaciones de Nopalea cochellinifera (L.) Salm-Dyck (Cactaceae) en Tamaulipas, México. Acta Universitaria, (29), pp. 1-11. http://doi.org/10.15174/au.2019.2238

Ochatt, S.J., Patat-Ochatt, E.M. and Moessner, A. 2011. Ploidy level determination within the context of in vitro breeding. Plant Cell, Tissue and Organ Culture (PCTOC), 104(3), pp. 329-341. https://doi.org/10.1007/s11240-011-9918-6

Oliveira, P.J., Palencia, G.P. and González, T.J. 2017. Caracterización de germoplasma de Agrostis y Festucas de los grupos rubra y ovina del norte de España. Pastos, 46(2), pp. 45-60.

Quero, C.A.R., Savidan, Y.H., Berthaud, J., Pérez, P.J., Espinoza, V.J. 1997. Estudio citogenético en el género Tripsacum. Agrociencia, 31, pp. 331-334.

Quero, C.A.R., Enríquez-Quiroz, J.F., Morales-Nieto, C.R. and Miranda-Jiménez, L. 2010. Apomixis y su importancia en la selección y mejoramiento de gramíneas forrajeras tropicales. Revisión. Revista Mexicana de Ciencias Pecuarias, 1(1), pp. 25-42. http://www.scielo.org.mx/pdf/rmcp/v1n1/v1n1a3.pdf

Riordan T.P., Fei S., Johnson P.G. and Read P.E. Plant breeding, plant regeneration, and flow cytometry in buffalograss. 1998. pp. 183–193. In Sticklen, M.B. and Kenna, M.P. (eds.) Turfgrass biotechnology: Cell and molecular genetic approaches to turfgrass improvement. Ann Arbor Press, Chelsea, MI.

Scaglia, J.A., Flores, D.G., Martinelli, M. 2021. Productivity of natural grasslands in different geomorphological units of the Sierras Pampeanas of Argentina. Ecosistemas, 30(2), pp. 2104.

Serrato-Cruz, M.A., Hernández-Rodríguez, M., Savidan, Y. and Bárcenas-Ortega, N.M. 2000. DNA content and ploidy level in Tagetes spp. using flow cytometry. Agrociencia, 34(6), pp. 729-734.

Soreng, R.J., Peterson, P.M., Romaschenko, K., Davidse, G., Teisher, J.K., Clark, L.G., Barberá, P., Gillespie, L.J.and Zuloaga, F.O. 2017. A worldwide phylogenetic classification of the Poaceae (Gramineae) II: An update and a comparison of two 2015 classifications. Journal of Systematics and Evolution, 55(4), pp. 259-290. https://doi.org/10.1111/jse.12262

Triana, M.Y. and Marsán, S.V. 2019. Aplicación de la citometría de flujo en el estudio de los síndromes linfoproliferativos crónicos. Instituto de Hematología e Inmunología. La Habana, Cuba. Revista Cubana de Hematología, 36(1), pp. 1137.

Villaseñor, J.L. 2016. Catálogo de las plantas vasculares nativas de México. Revista Mexicana de Biodiversidad, 87(3), pp. 559-902. https://doi.org/10.1016/j.rmb.2016.06.017

Wilkinson, M.J. and Stace, C.A. 1991. A new taxonomic treatment of the Festuca ovina L. aggregate (Poaceae) in the British Isles. Botanical Journal of the Linnean Society, 106(4), pp. 347-397. https://doi.org/10.1111/j.10958339.1991.tb02298.x




URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v29i1.66677

DOI: http://dx.doi.org/10.56369/tsaes.6667

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Copyright (c) 2026 Evert Villanueva Sánchez, Edgar Pozos-Méndez Eduardo Pozos Méndez, Martha Hernández Rodríguez, Adrián Raymundo Quero Carrillo, Hugo Alejandro Álvarez Hernández

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