In vitro GERMINATION AND INDUCTION OF MORPHOGENETIC RESPONSES IN PITAHAYA (Hylocereus undatus (HAW.) BRITTON & ROSE)

Nadia Guadalupe Sánchez-Coello, Luis Carlos Ortega-Macareno, Mauricio Luna-Rodríguez, Alejandro Salinas-Castro, Dulce María Murrieta-Hernández, Daniel Lopez-Lima

Abstract


Background. Hylocereus undatus is a plant of great economic importance that produces an edible fruit known as Pitahaya or dragon fruit. However, these methods result in a reduced genetic base and low germination rates, in addition to the low germination of seeds, which makes successful cultivation difficult. Objective. To establish an efficient procedure for disinfecting and germinating H. undatus seeds, laying the groundwork for future genetic improvement programs. Methodology. In vitro conditions, cladodes, cotyledons leaves, and stems were used as explants and cultured in MS medium with varying concentrations of ANA and BAP (0.5 mgL-1, 1.0 mgL-1, 1.5 mgL-1, 2.0 mgL-1, 2.5 mgL-1). Results. An 86% germination rate using MS medium without sucrose and a 47% germination rate in MS medium with added sucrose (30 gL-1). In the induction stage of morphogenetic responses, it was observed that the highest proliferation and shoot formation occurred when ANA was added at a concentration of 2.0 mgL-1 in the MS medium. Implications. It is necessary to lay the foundations for future genetic improvement programs aimed at increasing the plant material available to Pitahaya producers in Mexico. Conclusions. The best responses were seen in the cladodes, resulting in a greater number of shoots per explant.

Keywords


cacti; in vitro culture; morphogenesis.

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References


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URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v28i2.60335

DOI: http://dx.doi.org/10.56369/tsaes.6033

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