DOSE AND IMBIBITION TIMES OF HYDROGEN PEROXIDE IN GERMINATION AND INITIAL GROWTH OF COTTON (Gossypium barbadense L.)]

Arturo Morales Pizarro, Doris Rondoy-Castro, Pierina Escobedo-Huancas, Esther Durante-Montejo, Angie Veramatus-Mendoza, Jonathan Juárez-Vílchez, Henry Morocho-Romero, Roger Chanduvi-García, Marcos Quiroz-Calderón, Mariano Calero-Merino, Ricardo Peña-Castillo

Abstract


Background: Cotton is a crop of great socioeconomic interest in the textile industry in Peru, however, it is sown directly in the field where it presents irregular germination. Objective: To evaluate the effect of four doses of hydrogen peroxide and two imbibition times on the germination and initial growth of cotton. Methodology: The commercial hydrogen peroxide CP (H2O2 at a concentration of 3 g 100 mL-1) was used in the treatments: T0 (control- distilled water), T1 (10% CP), T2 (30% CP), T3 (50% CP), T4 (100% CP) subjected to 6 and 12 h of imbibition. Germination evaluations were made: germination percentage (GP), germination speed (GS), germination index (GI), Biometry: seed weight (SW), stem length (ST), number of leaves (NL) and root length (RL). Stem biomass: fresh weight (SFW) and dry weight (SDW), and root biomass: fresh weight (RFW) and dry weight (RDW). Results: T3-6 h improved germination variables. T1-6 h improved biometry and biomass variables. SW presented a negative correlation with NL. NL was positively correlated with SDW. SDW was positively correlated with SFW. Also, SFW with RL, RDW and RFW. RL with RDW. RDW with RFW and ST. The RFW with ST. Implications: The use of H2O2 increases germination and biomass of cotton seedlings. Conclusion: T3-6 h improved germination variables. However, T1-6 h improved biometry and biomass variables.

Keywords


cotton; biometry; germination; hydrogen peroxide; seedlings.

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References


Agraria., 2022. Exportaciones peruanas de algodón alcanzaron los US$ 1.66 millones en 2021, mostrando un incremento de 39.7%. https://www.agraria.pe/index.php/noticias/exportaciones-peruanas-de-algodon-alcanzaron-los-us-1-66-mil-27218#:~:text=En%202021%20las%20exportaciones%20de,39.7%25%20respecto%20al%20a%C3%B1o%20previo

Arroyo, E., De Navascues, P., Gómez-Ramírez, A., Molina, R., Perea, Á., García, J.L. and López-Santos, C., 2021. Factors triggering germination in plasma-activated cotton seeds: water imbibition vs. reactive species’ formation. Journal of Physics D: Applied Physics, 54(32), pp. 325205. https://iopscience.iop.org/article/10.1088/1361-6463/abfefc/meta

Bahin, E., Bailly, C., Sotta, B., Kranner, I., Corbineau, F. and Leymarie, J., 2011. Crosstalk between reactive oxygen species and hormonal signalling pathways regulates grain dormancy in barley. Plant, Cell And Environment, 34(6), pp. 980-993. https://doi.org/10.1111/j.1365-3040.2011.02298.x

Bailly, C., El-Maarouf-Bouteau, H. and Corbineau, F., 2008. From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. Comptes Rendus Biologies, 331(10), pp. 806-814. https://doi.org/10.1016/j.crvi.2008.07.022

Barba?Espin, G., Diaz?Vivancos, P., Clemente?Moreno, M.J., Albacete, A., Faize, L., Faize, M. and Hernández, J.A., 2010. Interaction between hydrogen peroxide and plant hormones during germination and the early growth of pea seedlings. Plant, Cell & Environment, 33(6), pp. 981-994. https://doi.org/10.1111/j.1365-3040.2010.02120.x

Batista, T.B., Binotti, E.D.C., Binotti, F.F.D.S., Sá, M.E.D. and Silva, T.A.D., 2018. Priming of brachiaria seeds with different sugar sources and concentrations. Revista Caatinga, 31, pp. 843-849. https://doi.org/10.1590/1983-21252018v31n406rc

Bewley, J.D., Bradford, K.J., Hilhorst, H.M.W. and Nonogaki, H., 2013. Seeds: Physiology of Development, Germination and Dormancy. New York, NY: Springer. https://doi.org/10.1017/S0960258513000287

Bhanuprakash, K. and Yogeesha, H. S., 2016. Seed priming for abiotic stress tolerance: an overview. Abiotic Stress Physiology of Horticultural Crops, pp. 103-117. https://doi.org/10.1007/978-81-322-2725-0_6

Cardoza-Viera, A., Arévalo-Valladolid, D., Javier-Alva, J., Peña-Castillo, R., Chanduví-García, R., Quiroz-Calderón, M. and Morales-Pizarro, D.A., 2024. Sustratos Orgánicos Alternos en la Germinación y Crecimiento Inicial de Plántulas de Maíz (Zea mays L.) en Condiciones de Vivero. Revista Terra Latinoamericana, 42. https://doi.org/10.28940/terra.v42i0.1867

Chakma, S.P., Chileshe, S.M., Thomas, R. and Krishna, P., 2021. Cotton seed priming with brassinosteroid promotes germination and seedling growth. Agronomy, 11(3), pp. 566. https://doi.org/10.3390/agronomy11030566

De Groot, G.J., Hundt, A., Murphy, A.B., Bange, M.P. and Mai-Prochnow, A., 2018. Cold plasma treatment for cotton seed germination improvement. Scientific Reports, 8(1), pp. 14372. https://doi.org/10.1038/s41598-018-32692-9

Dolatabadian, A. and Sanavy, S.A.M.M., 2008. Effect of the ascorbic acid, pyridoxine and hydrogen peroxide treatments on germination, catalase activity, protein and malondialdehyde content of three oil seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 36(2), pp. 61-66. http://dx.doi.org/10.15835/nbha36270

Dong, Z., Huang, J., Qi,, T., Fu, Q., Meng, A. and Fu, Y., 2023. Effects of Plant Regulators on the Seed Germination and Antioxidant Enzyme Activity of Cotton under Compound Salt Stress. Plants, 12(24), pp. 4112. https://doi.org/10.3390/plants12244112

Dos Santos-Guaraldo, M.M., Pereira, T.M., Dos Santos, H.O., De Oliveira, T.L., Pereira, W.V.S. and Von Pinho, E. V.D.R., 2023. Priming with sodium nitroprusside and hydrogen peroxide increases cotton seed tolerance to salinity and water deficit during seed germination and seedling development. Environmental and Experimental Botany, 209, pp. 105294. https://doi.org/10.1016/j.envexpbot.2023.105294

Dos Santos, I.S., de Jesús, O.N., Sampaio, S.R., Gonçalves, Z.S., Soares, T.L., De Santana Ferreira, J.R. and Lima, L.K.S., 2024. Salt tolerance strategy in passion fruit genotypes during germination and seedling growth and spectrophotometric quantification of hydrogen peroxide (H2O2). Scientia Horticulturae, 338, pp.113818. https://doi.org/10.3390/seeds1020008

Escobar-Álvarez, J.L., Ramírez-Reynoso, O., Saguilán P.C., Gutiérrez-Dorado, R., De los Ángeles Maldonado-Peralta, M. and Valenzuela-Lagarda J.L., 2021. Viabilidad y germinación en semillas de maíz criollo del estado de Guerrero. Ecosistemas y Recursos Agropecuarios, 8(2), pp. 1-10. https://doi.org/10.19136/era.a8nII.2963

Galecio-Julca, M., Neira-Ojeda, M., Chanduvi-García, R., Peña-Castillo, R., Álvarez-Bernaola, L. A., Granda-Wong, C. and Morales-Pizarro, A., 2023. Efecto de la eficacia de los microorganismos nativos y la composta en tres pisos altitudinales en el cultivo de quinua (Chenopodium quinoa) variedad INIA 415-Pasankalla. Terra Latinoamericana, 41. https://doi.org/10.28940/terra.v41i0.1622

Hajihashemi, S., Skalicky, M., Brestic, M. and Pavla, V., 2020. Cross-talk between nitric oxide, hydrogen peroxide and calcium in salt-stressed Chenopodium quinoa Willd. At seed germination stage. Plant Physiology and Biochemistry, 154, pp. 657-664. https://doi.org/10.1016/j.plaphy.2020.07.022

Hussain, A., Zahir, Z.A, Ditta, A., Tahir, M. U, Ahmad, M., Mumtaz, M.Z. and Hussain, S., 2019. Production and Implication of Bio-Activated Organic Fertilizer Enriched with Zinc-Solubilizing Bacteria to Boost up Maize (Zea mays L.) Production and Biofortification under Two Cropping Seasons. Agronomy, 10 (1), pp. 39. https://doi.org/10.3390/agronomy10010039

Katzman, L.S., Taylor, A. G. and Langhans, R.W., 2001. Seed Enhancements to Improve Spinach Germination. HortScience, 36(5), pp. 979-981. https://doi.org/10.21273/HORTSCI.36.5.979

Kong, X., Luo, Z., Zhang, Y., Li, W. and Dong, H., 2017. Soaking in H2O2 regulates ABA biosynthesis and GA catabolism in germinating cotton seeds under salt stress. Acta Physiologiae Plantarum, 39, pp. 1-10. http://dx.doi.org/10.1007/s11738-016-2299-z

López Medina, S.E., Mostacero León, J., Quijano Jara, C.H., Gil Rivero, A.E. and Rabanal Che Leon, M.F., 2020. caracterización del fruto semilla y fibra de Gossypium raimondii Ulbrich, ecotipo algodón silvestre. Ciencia y Tecnología Agropecuaria, 21(1), pp. 11-18. https://doi.org/10.21930/rcta.vol21_num1_art:1219

Maguire, J.D., 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2, pp. 176-177. DOI: http://dx.doi.org/10.2135/cropsci1962.0011183X000200020033x

Martínez-Reina, A. M, Hernández, B. and María J., 2015. La competitividad del algodón colombiano frente a los principales países productores mediante el enfoque de costos de producción. Ciencia y Tecnología Agropecuaria, 16(2), pp. 189-215. Retrieved January 09, 2023, from http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-87062015000200005&lng=en&tlng=es.

Mei, Y. and Song, S. 2008. Early morphological and physiological events occurring during germination of maize seeds. Agricultural Sciences in China, 7(8), pp. 950-957. DOI: http://dx.doi.org/10.1016/S1671-2927(08)60134-0

Merino-Valdés, M., Andrés-Meza, P., Leyva-Ovalle, O.R., López-Sánchez, H., Murguía-González, J., Núñez-Pastrana, R. and Luis, J., 2018. Influencia de tratamientos pregerminativos en semillas de chile manzano (Capsicum pubescens Ruiz & Pav.). Acta Agronómica, 67(4), pp. 531-537. https://doi.org/10.15446/acag.v67n4.73426

Mhamdi, A. and Van Breusegem, F. 2018. Reactive oxygen species in plant development. Development, 145(15), pp. dev164376. https://doi.org/10.1242/dev.164376

MINAM (Ministerio del ambiente)., 2020. Línea de base de la diversidad del algodón peruano con fines de bioseguridad. https://cdn.www.gob.pe/uploads/document/file/1490457/Libro%20en%20PDF.pdf?v=1608670524

Morales, G., y Camacho, M., 1985. Formato y recomendaciones para evaluar germinación. II Reunión Nacional sobre plantaciones Forestales. INIF/SARH Publicación Especial, 48.

Morales-Pizarro, A., Rivas-Chero, A.A., Zapata-Córdova, A.C., García-Guevara, E., Ruesta-López, M. and Peña-Castillo, R., 2023. Efecto de diferentes dosis de ácido giberélico en la germinación de papaya (Carica papaya L.) variedad criolla. Chilean Journal of Agricultural & Animal Sciences, 39(3), pp. 392-400. https://doi.org/10.29393/CHJAA39-35EDAM60035

Ozaki, K., Uchida, A., Takabe, T., Shinagawa, F., Tanaka, Y., Takabe, T. and Takabe, T., 2009. Enrichment of sugar content in melon fruits by hydrogen peroxide treatment. Journal of plant physiology, 166(6), pp. 569-578. https://doi.org/10.1016/j.jplph.2008.08.007

Paiva, E., Lemos-Filho, J. and Oliveira, D., 2006. Imbibition of Swietenia macrophylla (Meliaceae) seeds: the role of stomata. Annals of Botany, 98(1), pp. 213-217. DOI: http://dx.doi.org/10.1093/aob/mcl090

R Core Team., 2020. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing.

Raphael, J.P., Gazola, B., Nunes, J.G., Macedo, G.C. and Rosolem, C.A., 2017. Cotton germination and emergence under high diurnal temperatures. Crop Science, 57(5), pp. 2761-2769. https://doi.org/10.2135/cropsci2017.03.0182

Reyes-More, P.M. 2014. El algodón Pima peruano: Cultivo y manejo agronómico. https://repositorio.unp.edu.pe/bitstream/handle/UNP/1139/Libro%20Algodon.pdf

Romero-Romero, M.T. and López-Delgado, H.A., 2009. Ameliorative effects of hydrogen peroxide, ascorbate and dehydroascorbate in Solanum tuberosum infected by phytoplasma. American Journal of Potato Research, 86, pp. 218-226. https://doi.org/10.1007/s12230-009-9075-1

Ruesta-López, M., Zurita-Chinguel, L., Lizano-Pintado, M., Delgado-Vite, M., Zapata-Durand, D., Jiménez-Castillo, J., Peña-Castillo, R., Galecio-Julca, M., Chanduví-García, R., & Morales Pizarro, D., 2024. Biostimulant and imbibition times on passion fruit (Passiflora edulis s., passifloraceae) seed germination. Tropical and Subtropical Agroecosystems, 27(2), p.076. http://dx.doi.org/10.56369/tsaes.5199

Santhy, V., Meshram, M., Wakde, R. and Vijaya-Kumari, P.R., 2014. Hydrogen peroxide pre-treatment for seed enhancement in cotton (Gossypim hirsutum L.). African Journal of Agricultural Research, 9(25), pp. 1982-1989. http://dx.doi.org/10.5897/AJAR2013.7210

Scott, S., Jones, R. and Williams, W., 1984. Review of data analysis methods for seed germination. Crop Science, 24, pp. 1129-1199. https://www.researchgate.net/profile/AlmaOrozcoSegovia/publication/284666517_Metodos_de_analisis_de_datos_en_la_germinacion_de_semillas_un_ejemplo_Manfreda_brachystachya/links/56df0cfa08aee77a15fcefcf/Metodosde-analisis-de-datos-en-la-germinacionde-semillas-un-ejemplo-Manfredabrachystachya.pdf

Tania, S.S., Rahaman, M.M., Rauf, F., Suborna, M.A., Humayun Kabir, M., Hoque, M.A. and Rhaman, M.S., 2021. Seed priming with salicylic acid (SA) and hydrogen peroxide (H2O2) improve germination and seedling growth of wheat (Triticum aestivum) under salt stress. Asian Journal of Research in Crop Science, 6(4), pp.60-69. https://doi.org/10.9734/ajrcs/2021/v6i430127

Veloso, L.L.D.S.A., Capitulino, J.D., De Azevedo, C.A.V., Da Silva, A.A.R. and Gheyi, H.R., 2021. Application methods of hydrogen peroxide in soursop seedlings irrigated with saline water. Comunicata Scientiae, 12, pp.1-8. https://doi.org/10.14295/cs.v12.3288

Wang, M., van der Meulen, R.M., Visser, K., Van Schaik, H.P., Van Duijn, B. and de Boer, A.H., 1998. Effects of dormancy-breaking chemicals on ABA levels in barley grain embryos. Seed Science Research, 8(2), pp.129-137. http://doi.org/10.1017/S0960258500004025

Wojtyla, ?., Lechowska, K., Kubala, S. and Garnczarska, M., 2016. Different modes of hydrogen peroxide action during seed germination. Frontiers in Plant Science, 7, pp. 66. https://doi.org/10.3389/fpls.2016.00066

Xiao, S., Liu, L., Wang, H., Li, D., Bai, Z., Zhang, Y. and Li, C., 2019. Exogenous melatonin accelerates seed germination in cotton (Gossypium hirsutum L.). PloS one, 14(6), pp. e0216575. https://doi.org/10.1371/journal.pone.0216575

Yao, X., Zhou, M., Ruan, J., Peng, Y., Yang, H., Tang, Y. and Cheng, J., 2021. Pretreatment with H2O2 alleviates the negative impacts of NaCl stress on seed germination of Tartary buckwheat (Fagopyrum tataricum). Plants, 10(9), pp. 1784. https://doi.org/10.3390/plants10091784




URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v28i1.56606

DOI: http://dx.doi.org/10.56369/tsaes.5660



Copyright (c) 2025 Arturo Morales Pizarro, Doris Rondoy-Castro, Pierina Escobedo-Huancas, Esther Durante-Montejo, Angie Veramatus-Mendoza, Henry Morocho-Romero, Roger Chanduvi-García, Marcos Quiroz-Calderón, Mariano Calero-Merino, Ricardo Peña-Castillo

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