Evaluation of pregerminative treatments in seeds of Malpighia glabra L.

Monserrat Concepción Esquivel Chi, Rubén Humberto Andueza Noh, Rene Garruña, Angel Manuel Herrera Gorocica, Esaú Ruíz Sánchez, Marcela Gamboa, Daniel Potter, Emanuel Hernández Nuñez

Abstract


Background. Malpighia glabra is a native fruit species, with great nutritional and economic potential to which several biological activities are attributed. However, it presents serious problems related to its germination. Objective. To evaluate the physiological response of M. glabra seeds and seedlings subjected to four pregermination treatments. Methodology. The pregerminative treatments used were: 1) control (water), 2) immersion in water at 60°C for 5 min, 3) immersion in water for 48 h, 4) immersion in gibberellic acid (AG3) for 48 h. Germination, emergence and growth of seedlings were evaluated up to 45 days. Results. Immersion in AG3 caused delays in germination, but had a positive effect on the size and dry weight of the leaves. The treatments that resulted in the highest emergence value were water immersion with 45% and hot water immersion with 40%, they also resulted the highest values in dry weight of stem and root. Implication. Pregerminative treatments are an accessible and economical option to improve the germination of M. glabra seeds. Conclusion. The treatments did not influence final germination percentage. However, water immersion and hot water immersion obtained the highest percentages of emergence and dry weight of stem and root.

Keywords


acerola; plant genetic resource; thermal shock; seedlings.

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References


Alcántara-Cortes, J., Acero, G.J., Alcántara, C.J. and Sánchez, M.R., 2019. Principales reguladores hormonales y sus interacciones en el crecimiento vegetal. NOVA, 17(32), pp. 109–129. https://doi.org/10.25058/24629448.3639

Allen, E., Alvarez, S., 2020. International Rules for Seed Testing 2020; The International Seed Testing Association: Bassersdorf, Switzerland.

Araújo, P.S.R. and Minami, K., 1994. Acerola. Campinas, Fundacao Cargill. São Paulo, Brasil.

Azerêdo, G.A., Matos, V.P., Germano, M.L.A.R. and Lima, A.A., 1994. Efeito da temperatura e períodos de embebição na germinação de sementes de acerola (Malpighia glabra L.). In: XIII Congreso Brasileiro de Fruticultura. Salvador de Bahía, Brasil. pp. 68–69.

Azerêdo, G.A., Matos, P.V., Lima, A.A., da Silva, A. and Guedes, M.A., 2006. Viabilidade de sementes de acerola (Malpighia punicifolia DC) influenciada pelo sustrato, temperatura e coloracae de frutos. Pesquisa Agropecuária Tropical, 36(1), pp. 7–11.

Azerêdo, G.A., Matos, P.V., Lopes, P.K., da Silva, A. and Rodrigues, de F.L., 2005. Viabilidade e vigor de sementes de acero¬la (Malpighia punicifolia) submetidas à embebição sob diferentes temperaturas. Pesquisa Agropecuária Tropical, 35(2), pp. 81–84.

Birchler, T.A., Rose, R.W., Royo, A., and Pardos, M., 1998. La planta ideal: Revisión del concepto, parámetros definitorios e implementación práctica. Investigación Agraria, Sistemas y Recursos Forestales, 7(1–2), pp. 109–121.

Chowdhury, A., Yonemoto, Y., Kato, H. and Macha, M., 2005. Classification of some acerola (Malpighia glabra Linn.) cultivars using morphometric descriptors and RAPD markers. Japanese Journal of Tropical Agriculture, 49(4), pp. 255–263.

Cornea-Cipcigan, M., Pamfil, D., Sisea, C.R., M?rg?oan, R., 2020. Gibberellic acid can improve seed germination and ornamental quality of selected cyclamen species grown under short and long days. Agronomy, 10(4), pp. 516. https://doi.org/10.3390/agronomy10040516

Corrêa, C.V., Gouveia, A.M.D.S., Martins, B.N., Jorge, L.G., Lanna, N., Tavares, A.E.B., Mendonça, V.Z. and Evangelista, R.M., 2017. Influence of ripening stages on physicochemical characteristics of acerola fruits. Revista de Ciências Agrárias, 40(4), pp. 808–813. https://doi.org/10.19084/RCA17116

Costa, L.C., Pavani, M.D.C.M., Moro, F.V., and Perecin, D., 2003. Viabilidade de sementes de acerola (Malpighia emarginata D.C.): avaliação da vitalidade dos tecidos. Revista Brasileira de Fruticultura, 25(3), pp. 532–534. http://dx.doi.org/10.1590/S0100-29452003000300043

Da Silva Nunes, R., Silva Kahl, V.F., Da Silva Sarmento, M., Richter, M.F., Abin-Carriquiry, J.A., Martinez, M. M., De Barros Falcão Ferraz, A. and Da Silva, J., 2013. Genotoxic and antigenotoxic activity of acerola (Malpighia glabra L.) extract in relation to the geographic origin. Phytotherapy Research, 27(10), pp. 1495–1501. https://doi.org/10.1002/ptr.4896

De Assis, S., Fernandes, F.P., Martins, A. and De Faria Oliveira, O., 2008. Acerola: Importance, culture conditions, production and biochemical aspects. Fruits, 63(2), pp. 93–101. http://doi.org/10.1051/frutas:2007051

Di Rienzo, J.A., Casanoves, F., Gonzales, L.A., Tablada, E.M., Diaz, M.P., Robledo, C.W., and Balzarini, M.G., 2018. InfoStat ver. 2018. Grupo InfoStat, FCA Universidad Nacional de Cordoba, Argentina.

Dias, P.M.B., Brunel-Muguet, S., Dürr, C. Huget, T., Demilly, D., Wagner, M.-H., and Teulat-Merah, B., 2011. QTL analysis of seed germination and pre-emergence growth at extreme temperatures in Medicago truncatula. Theoretical and Applied Genetics, 122, pp. 429–444. https://doi.org/10.1007/s00122-010-1458-7

Dickson, A., Leaf, A.L. and Hosner, J.F., 1960. Quality appraisal of white spruce and white pine seedling stock in nurseries. The Forestry Chronicle, 36(1), pp.10–13. https://doi.org/10.5558/tfc36010-1

Dutta, P., 2018. Seed priming: New vistas and contemporary perspectives. In: Rakshit, A., Singh, H. (eds) Advances in seed priming. Springer, Singapore. https://doi.org/10.1007/978-981-13-0032-5_1

García-Hoyos, A., Sánchez-Robles, J., García-Hernández, L.A. and León-González, F.D., 2011. Reproducción sexual e influencia de sustratos en el desarrollo de Malpighia glabra L. (Malpighiaceae). Polibotánica, 32, pp. 119–133. https://www.polibotanica.mx/index.php/polibotanica/article/view/841

Garruña-Hernández, R., Orellana, R., Larque-Saavedra, A. and Canto, A., 2014. Understanding the physiological responses of a tropical crop (Capsicum chinense Jacq.) at high temperature. PLOS ONE, 9(11), pp. e111402. https://doi.org/10.1371/journal.pone.0111402

Germano, M.L.A.R., Matos, V.P., Azerêdo, G.A. and Lima, A.A., 1994. Influência de diferentes substratos na germinação de sementes de acerola (Malpighia glabra L.). In: Congresso Brasileiro de Fruticultura. Salvador. pp. 70–71.

Gomes, J.E., 2001. Aspectos botânicos, físicos-químicos, genéticos e influências meteorológicas em aceroleiras (Malpighia emarginata D C.) no processo seletivo de genótipos de Itápolis, Viradouro e Jaboticabal, sp, Ph.D. Faculdade de Ciências Agrárias e Veterinárias, Universidad Estadual Paulista, Jaboticabal.

Grzesik, M., Górnik, K., Janas, R., Lewandowki, M., Romanowska-Duda, Z. and van Duijn, B., 2017. High efficiency stratification of apple cultivar Ligol seed dormancy by phytohormones, heat shock and pulsed radio frequency. Journal of Plant Physiology, 219, pp. 81–90. https://doi.org/10.1016/j.jplph.2017.09.007

Hoang, Q.B., Pham, N.T., Le, T.T. and Duong, T.N.D., 2022. Bioactive compounds and strategy processing for acerola: A review. Can Tho University Journal of Science, 14(2), pp. 46–60. https://doi.org/10.22144/ctu.jen.2022.011

Jiménez-Osornio, J., Pastrana, D., Molina, A., Ruenes, M. del R., Montañez, P. and Lendechy, Á., 2018. Sustainable agriculture through resurrecting indigenous fruits in Yucatán. In C. Scherrer & S. Verma (Eds.), Decent work deficits in southern agriculture: measurements, drivers and strategies. Rainer Hampp Verlag. pp. 305–322.

Kumar, S., Bhajipale, N.S., Sanghai, D.B. and Padgilwar, S.S., 2017. Phytochemical and pharmacological overview of acerola cherry: a review. International Journal of Research in AYUSH and Pharmaceutical Sciences IJRAPS, 1(1), pp. 2456–9909.

Lacerda, V.R., Pagehú, L.F., Gonçalves, A.P., Vieites, R.L., Lopes, P.S.N., 2022. Gibberellic acid concentrations and storage of Caryocar brasiliense (Caryocaraceae) seeds propagated in tubes. Horticulturae, 8(11), pp. 1094. https://doi.org/10.3390/horticulturae8111094

Laskowski, L. and Bautista, D., 2002. Efecto de la escarificación y profundidad de siembra sobre la germinación y emergencia de Malpighia emarginata DC. Bioagro, 14 (2), pp. 77–83.

Laskowski, L. and Bautista, D., 2003. Estudio fenológico del crecimiento y desarrollo de la plántula de semeruco Malpighia emarginata DC. Bioagro, 15(3), pp. 183–191.

Ledea-Rodríguez, J.L., Reyes-Pérez, J.J., Castellanos, T., Angulo, C., Reynoso-Granados, T., and Alcaraz-Melendez, L., 2020. Crecimiento, desarrollo y calidad de plántulas de Moringa oleifera (Lamark) inoculadas con bacterias promotoras del crecimiento vegetal. Tropical and Subtropical Agroecosystems, 23 (3), pp. 74. https://doi.org/10.56369/tsaes.3280

Lim, T.K., 2012. Malpighia emarginata. (Ed.) Lim, T. K. In: Edible medicinal and non-medicinal plants. Netherlands. Springer, Dordrecht. 3, pp. 153–159.

Maldonado Peralta, M.A., García De Los Santos, G., García-Nava, J.R., Corona-Torres, T., Cetina-Alcalá, V. M., and RamírezHerrera, C., 2016. Calidad morfológica de frutos y endocarpios de nanche rojo (Malpighia mexicana, Malpighiaceae). Acta Botánica Mexicana, 117, pp. 37–46. https://doi.org/10.21829/abm117.2016.1166

Mattana, E., Sacandé, M., Abdul Sanogo, K., Lira, R., Gómez-Barreiro, P., Rogledi, M. and Ulián, T., 2017. Thermal requirements for seed germination of underutilized Lippia species. South African Journal of Botany,109, pp. 223–230. https://doi.org/10.1016/j.sajb.2016.12.020

Menezes, T.P., Rufini, J.C.M., Pio, L.A.S., Magalhaes, D.S., and Chain, C.P., 2019. Physical and chemical characterization of fruits and DNA content in genotypes of acerola tree. Brazilian Journal of agriculture, 93(3), pp. 336–348. https://doi.org/10.37856/bja.v93i3.3323

Mezadri, T., Villaño, D., Fernández-Pachón, M.S., García-Parrilla, M.C. and Troncoso A.M., 2008. Antioxidant compounds and antioxidant activity in acerola (Malpighia emarginata DC.) fruits and derivatives. Journal of Food Composition and Analysis, 21(4), 282–290. https://doi.org/10.1016/j.jfca.2008.02.002

Nascimento, E.M.M., Rodrigues, F.F.G., Costa, W.D., Teixeira, R.N.P., Boligon, A.A., Sousa, E.O., Rodrigues, F.F.G., Coutinho, H.D.M., and da Costa, J.G.M., 2018. HPLC and in vitro evaluation of antioxidant properties of fruit from Malpighia glabra (Malpighiaceae) at different stages of maturation. Food and Chemical Toxicology, 119, pp. 457–463. https://doi.org/10.1016/j.fct.2017.11.042

Nonogaki, H., Barrero, J.M., and Li, C., 2018. Seed dormancy, germination, and pre-harvest sprouting. Frontiers in Plant Science, 9, pp. 1783. https://doi.org/10.3389/fpls.2018.01783

Palepad, K.B., Bharad, S.G. and Bansode, G.S., 2017. Effect of seed treatments on germination, seedling vigour and growth rate of custard apple (Annona squamosa). Journal of Pharmacognosy and Phytochemistry, 6(5), pp. 20–23.

Paz Paz, M., Rodríguez Trejo, D.A., Villanueva Morales, A., De la Rosa, B., and Máxima, M.A., 2023. Fertilization, plant quality and field survival of Pinus spp. in Ixtlán de Juárez, state of Oaxaca. Revista Mexicana de Ciencias Forestales, 14(76), pp. 71-92. https://doi.org/10.29298/rmcf.v14i76.1324

Pompelli, M.F., Jarma-Orozco, A., and Rodriguez-Páez, L.A., 2023. Imbibition and germination of seeds with economic and ecological interest: physical and biochemical factors involved. Sustainability, 15(6), pp. 5394. https://doi.org/10.3390/su15065394

Popinigis, F., 1985. Fisiologia da semente. Agiplan. Brasília.

Prakash, A. and Baskaran, R., 2018. Acerola, an untapped functional superfruit: a review on latest frontiers. Journal of Food Science and Technology, 55(9), pp. 3373–3384. https://doi.org/10.1007/s13197-018-3309-5

Rodríguez, T.D.A., 2008. Indicadores de calidad de planta forestal. México. Mundi Prensa. pp. 156

Rout, S., Beura, S., Khare, N., 2016. Effect of GA3 on seed germination of Delonix regia. Research Journal of Recent Sciences, 5(ISC-2015), pp.1-3.

Sánchez-Aguilar, H., Aldrete, A., Vargas-Hernández, J. and Ordaz-Chaparro, V., 2016. Influencia del tipo y color de envase en el desarrollo de plantas de pino en vivero. Agrociencia, 50 (4), pp. 481–492.

Sant’Ana, C. R. de O., Paiva, R., Reis, M. V. dos, Silva, D.P.C. da, and Silva, L.C., 2018. In vitro propagation of Campomanesia rufa: An endangered fruit species. Ciência e Agrotecnologia, 42(4), pp. 372–380. https://doi.org/10.1590/1413-70542018424011018

Sher, A., Sarwar, T., Nawaz, A., Ijaz, M., Sattar, A., and Ahmad, S., 2019. Methods of seed priming. In: Hasanuzzaman, M., Fotopoulos, V. (eds) Priming and pretreatment of seeds and seedlings. Springer, Singapore. https://doi.org/10.1007/978-981-13-8625-1_1

Silva-Correia, J., Freitas, S., Tavares, R.M. Azevedo, H., 2014. Phenotypic analysis of the Arabidopsis heat stress response during germination and early seedling development. Plant Methods, 10(7), pp. 1-11. https://doi.org/10.1186/1746-4811-10-7

Steel, R.G.D. and Torrie, J.H. 1980. Principles and procedures of statistics. 2aed. N.Y. Mc Graw Hill. pp. 481.

Taiz, L., Zeiger, E., Møller, I.M. and Murphy, A., 2017. Fisiología y Desenvolvimento Vegetal; Artmed Editora: Porto Alegre, Brasil.

Tapfumaneyi, L., Dube, P., Mavengahama, S., and Ngezimana, W., 2023. Effect of gibberellic acid and potassium nitrate seed treatments on the emergence and seedling vigor of Amaranth and Cleome gynandra. Agrosystems, Geosciences & Environment, 6, pp. e20359. https://doi.org/10.1002/agg2.20359

Tena-Meza, M., Navarro-Cerrillo, R.M. and Brizuela Torres, D., 2021. Distribution of Malpighia mexicana in Mexico and its implications for Barranca del Río Santiago. Journal of Forestry Research, 32(3), pp.1095–1103. https://doi.org/10.1007/s11676-020-01157-z

Thomas, S.G., Rieu, I., Steber, C.M., 2005. Gibberellin metabolism and signaling. Vitamins & Hormones, 72, pp. 289-338. https://doi.org/10.1016/s0083-6729(05)72009-4

Torres-Contreras, A.M., García-Baeza, A., Vidal-Limon, H.R., Balderas-Renteria, I., Ramírez-Cabrera, M.A., and Ramirez-Estrada, K., 2022. Plant secondary metabolites against skin photodamage: Mexican plants, a potential source of uv-radiation protectant molecules. Plants, 11(2), pp. 1–25. https://doi.org/10.3390/plants11020220

Tweddle, J.C., Dickie, J.B., Baskin, C.C. and Baskin, J.M., 2003. Ecological aspects of seed desiccation sensitivity. Journal of Ecology, 91: 294-304. https://doi.org/10.1046/j.1365-2745.2003.00760.x

Vásquez, W., Pupiales, P., Viteri, P., Sotomayor, A., Feicán, C., Campaña, D. and Viera, W., 2019. Chemical scarification and use of gibberellic acid for seed germination of blackberry cultivars (Rubus glaucus Benth). Interciencia, 44(3), pp. 159–164. https://www.interciencia.net/wp-content/uploads/2019/06/161_6331_Com_Viera_v44n3.pdf

Wang, J., Hui, D., Lu, H., Wang, F., Liu, N., Sun, Z., and Ren, H., 2019. Main and interactive effects of increased precipitation and nitrogen addition on growth, morphology, and nutrition of Cinnamomum burmanni seedlings in a tropical forest. Global Ecology and Conservation, 20, pp. e00734. https://doi.org/10.1016/j.gecco.2019.e00734

Wang, L.L., Chen, X.Y., Yang, Y., Wang, Z., Xiong, F., 2016. Effects of exogenous gibberellic acid and abscisic acid on germination, amylases, and endosperm structure of germinating wheat seeds. Seed Science and Technology, 44(1), pp. 64–76. https://doi.org/10.15258/sst.2016.44.1.09




URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v29i3.53156

DOI: http://dx.doi.org/10.56369/tsaes.5315

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