VARIETIES OF CHILE (Capsicum annuum L.) IDENTIFIED BY RAPD MARKERS

Abstract

In Mexico there is a great diversity of species and varieties of chile with great cultural, gastronomic and economic importance. The domesticated species Capsicum annuum groups most of the cultivated types and is the most important in Mexico and the world; it is important to consider the genetic richness, therefore, there is a need to study it through molecular techniques of genetic analysis. For that reason, the objective was to analyze C. annuum varieties of free pollination and creole varieties among themselves, using RAPD markers (random amplified DNA polymorphism), to measure the efficiency of OPA primers and the detection of polymorphisms. Six varieties of C. annuum of the "ancho" and "mirasol" types we used, classified in free pollination and criollas varieties. The varieties they submitted to a RAPD analysis with the initiators OPA 02, OPA 11 and OPA 20. The results showed little variability between the six varieties of C. annuum and the dendogram showed two main groups, classifying them according to their technological level. Therefore, the RAPD are a useful tool in the detection of variation and have great power for the estimation of the genetic similarities in C. annuum.