ISOLATION OF INOCUOUS MICROORGANISMS ABLE TO PRODUCE SIDEROPHORES USEFUL IN PHYTOREMEDIATION SYSTEMS

Abstract

Soil pollution reduces yields and food quality. Fitorremediation is a process used to recuperate polluted soils which competes favorably with more expensive physical and chemical methods. Samples of soil and roots of wild plants were collected at nine sites of Colegio de Postgraduados, Campus Montecillo. After determining the pH of soil samples, the supernatant was streaked on solid King's B medium. Root apex samples were macerated and the liquid collected was streaked on the same medium and all the dishes were incubated at 26 to 28 ° C. Of 223 bacterial isolates obtained, 23 were selected for producing fluorescent pigments and being safe for plants. Two soil (2S-10, 2S-9) and one root (2R-2) isolates had the most fluorescent pigment production. Alfalfa seeds inoculated with cells of the isolates 2S-10, 2R-2, and 2S-9 germinated 78, 56, and 88 %, respectively, while uninoculated seeds germinated 88 %. A preliminary test highlighted the best development of the inoculated seedlings with the isolate 2S10. Therefore, using plant-microbe interaction is possible to enhance the potential of plants to accumulate metals, making phytoremediation a more efficient process.