Biological effectiveness of Trichoderma spp. strains against guava scab disease (Pestalotiopsis clavispora) under in vitro and field conditions

Ernesto Gonzalez-Gaona, Karla Vanessa De Lira-Ramos, Olga Lidia Rivera-Dávila, Roberto Sanchez-Lucio, Lucila Perales-Aguilar, Efraín De La Cruz-Lázaro

Abstract


Background: Guava scab is a disease caused by Pestalotiopsis clavispora, and chemical agents are used for its control. An alternative is the use of antagonistic fungi such as Trichoderma spp., which has been extensively studied and has a low environmental impact. Objective: In this study, the biological effectiveness of Trichoderma strains against Pestalotiopsis sp. was evaluated. Methodology: Dual bioassays were established, where mycelial discs of Trichoderma and Pestalotiopsis were placed equidistantly on PDA medium to evaluate the antagonist's behavior against the pathogen. In the field, six Trichoderma strains, two fungicides, one insecticide, and the wateronly control were evaluated. At harvest, the incidence and severity of the disease, as well as the biological effectiveness of the treatments, were determined. Results: The bioassays showed that most Trichoderma strains are good competitors for colonizing the medium. They also showed mycoparasitism and reduced Pestalotiopsis growth. Implication: In the field, two Trichoderma strains achieved a reduction in the percentage of damaged fruit, lower severity, and greater effectiveness, similar to one of the fungicides and the insecticide. Conclusion: These strains can be considered in field level management for guava stem blight control, with a lower environmental impact.

Keywords


Biological control; antagonist fungus; dual bioassays; field evaluation.

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References


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URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v28i3.64510

DOI: http://dx.doi.org/10.56369/tsaes.6451

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Copyright (c) 2025 Ernesto Gonzalez-Gaona, Karla Vanessa De Lira-Ramos, Olga Lidia Rivera-Dávila, Roberto Sanchez-Lucio, Lucila Perales-Aguilar, Efraín De La Cruz-Lázaro

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