MINERAL AND CARBOHYDRATE ACCUMULATION AND THEIR RELATIONSHIP WITH MACRO- AND MICRO MORPHOLOGY OF Dasylirion cedrosanum TREL.

Emir Lenin Serafin-Higuera, Manuel Humberto Reyes-Valdés, Hermila Trinidad García-Osuna, América Berenice Morales-Díaz, José Ángel Villarreal-Quintanilla, Julia Medrano-Macías, Guillermina Barrientos-Rivera, Adalberto Benavides-Mendoza

Abstract


Background. Dasylirion cedrosanum Trel. is used to produce sotol, which has had an increase in its production. In view of this, producers are interested in establishing monocultures to mitigate its overexploitation. The lack of information on the ecophysiology of the plant hinders these measures. Objective. To analyze and associate the carbohydrate content, the micromorphological and macromorphological characters, and the mineral components of the stem and leaf of D. cedrosanum. Methodology. Leaf and stem tissue samples were collected from 10 plants in San Lorenzo, General Cepeda and Buenavista in Coahuila. Fructose, glucose, starch and mineral contents were determined, and macro and micromorphological character data were collected. Analysis was performed by ANOVA and Pearson's correlation. Results. Fructose content was higher in the stem and starch in the leaf. Plants from Buenavista presented a higher concentration of carbohydrates and minerals, and had the highest values for macromorphological characters. Micromorphological characters did not show significant differences. Correlations indicated a negative association of Cu and Pb with carbohydrates and micromorphology. In contrast, K, P and Mg were positively associated. Conclusions. Different conditions at each locality influenced macromorphology and concentrations of minerals, glucose starch and fructose. Implications. The information obtained is the basis for the establishment and management of nursery plants. 

Keywords


Adaptation to arid environments; arid zone plant ecophysiology; carbohydrate content; mineral concentration; agronomic management; sotol production.

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References


Aloni, R, 2021. Environmental adaptation of vascular tissues. In R Aloni, ed. Vascular differentiation and plant hormones. Springer, Cham. pp. 251-258. https://doi.org/10.1007/978-3-030-53202-4

Barrientos-Rivera, G., Hernández-Castro, E., Sampedro-Rosas, M.L. and Segura-Pacheco., H.R., 2020. Conocimiento tradicional y academia: productores de maguey y mezcal de pequeña escala en las regiones Norte y Centro de Guerrero, México. Sociedad y Ambiente, 23, págs. 1-28. https://doi.org/10.31840/sya.vi23.2173

Barrios, A.A., Ariza, F.R., Molina M.J.M., Espinosa, P.H. and Bravo, ME, 2006. Manejo de la fertilización de magueyes mezcaleros cultivados (Agave spp) de Guerrero. Iguala, Gro. México: Folleto Técnico Núm. 13. INIFAP. Campo Experimental Iguala.

Benavides-Mendoza, A., Hernández-Juárez, A. and Francisco-Francisco, N., 2023. Germinación, crecimiento vegetativo y morfología floral de Dasylirion cedrosanum del matorral rosetófilo de Coahuila. CienciaUAT, 18, pp.191-201. https://doi.org/10.29059/cienciauat.v18i1.1772

Bhadwal, S. and Sharma, S., 2022. Selenium alleviates carbohydrate metabolism and nutrient composition in arsenic stressed rice plants. Rice Science, 29, pp. 385-396. https://doi.org/10.1016/j.rsci.2021.11.008

Boxler-Baldoma, C., Lütz, C., Heumann, H.G. and Siefermann-Harms, D., 2006. Structural changes in the vascular bundles of light-exposed and shaded spruce needles suffering from Mg deficiency and ozone pollution. Journal of Plant Physiology, 163, pp. 195-205. https://doi.org/10.1016/j.jplph.2005.03.008

Canales, G.L.E. and Morán, R.F.I., 2017. Geología. In: D Quintanilla Rodríguez, FI Morán Rosales y KC Nájera Cordero, eds. La biodiversidad en Coahuila. Estudio de estado. Vol I. México: Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO), pp. 55-69.

Cano, P.A., Martínez, B.O.U., Berlanga, R.C.A., Villavicencio, G.E.E. and Castillo, Q.D., 2011. Guía para la evaluación de existencias de sotol (Dasylirion cedrosanum Trel.) en poblaciones naturales del estado de Coahuila. Saltillo, Coahuila México: Folleto Técnico Núm. 43. INIFAP. Campo Experimental Saltillo.

Cen-Cen, E.R., Gómez-Merino, F. and Martínez-Hernández, A., 2015. Tolerancia de Agave tequilana a altas concentraciones de cationes metálicos divalentes. Polibotánica, 40, pp. 163-182. http://doi.org/10.18387/polibotanica.40.11

Chu, K.L., Koley, S., Jenkins, L.M., Bailey, S.R., Kambhampati, S., Foley, K., Arp, J.J., Morley, S.A., Czymmek, K.J., Bates, P.D. and Allen, D.K., 2022. Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves. Metabolic Engineering, 69, pp. 231-248. https://doi.org/10.1016/j.ymben.2021.12.003

Collin, S., Baskar, A., Geevarghese, D.M., Ali, M.N.V.S., Bahubali, P., Choudhary, R., Lvov, V., Tovar, G.I., Senatov, F., Koppala, S. and Swamiappan, S., 2022. Bioaccumulation of lead (Pb) and its effects in plants: A review. Journal of Hazardous Materials Letters, 3. pp. 100064. https://doi.org/10.1016/j.hazl.2022.100064

Cooksey, R.W., 2020. Correlational statistics for characterizing relationships. In: Cooksey, R.W, ed. Illustrating statistical procedures: Finding meaning in quantitative data. Springer Nature, Singapore, pp. 141-239. https://doi.org/10.1007/978-981-15-2537-7

Ferreira, T. and Rasband, W., 2018. ImageJ userguide. Revised Edition. https://imagej.nih.gov/ij/docs/guide/index.html, consultado 24 de octubre de 2024.

Fick, K.R., Miller, S.M., Funk, J.D., McDowell, L.R. and Houser, R.H., 1976. Methods of Mineral Analysis for Plant and Animal Tissues. Segunda edición. Gainesville, Florida: Universidad de Florida.

Flores, S.I.R. and Castro, A.C., 2024. Distribución, riqueza, endemismo y conservación del género Dasylirion (Asparagaceae, Convallarioideae). Acta Botánica Mexicana, 131, pp. 1-22. https://doi.org/10.21829/abm131.2024.2367

Flores-Gallegos, A.C., Cruz-Requena, M., Castillo-Reyes, F., Rutiaga-Quiñones, O.M., Torre, L.S., Paredes-Ortíz, A. and Rodríguez-Herrera, R., 2019. Sotol, an alcoholic beverage with rising importance in the worldwide commerce. In: Mihai GA, Maria HA, eds. Alcoholic Beverages. Kidlington, Elsevier Reino Unido, pp. 141 160. https://doi.org/10.1016/B978-0-12-815269-0.00005-2

Francisco-Francisco, N., García-Osuna, H.T., Benavides-Mendoza, A., Hernández-Juárez, A. and Ramírez Godina, F., 2016. Morfología y anatomía foliar de Dasylirion cedrosanum en diferentes etapas de desarrollo. Revista Mexicana de Ciencias Agrícolas, 7, pp. 1679-1687. https://doi.org/10.29312/remexca.v7i7.161.

Guo, W., Nazim, H., Liang, Z. and Yang, D., 2016. Magnesium deficiency in plants: An urgent problem. The Crop Journal, 4, pp. 83-91. https://doi.org/10.1016/j.cj.2015.11.003

Harrison, E.L., Arce, C.L., Gray, J.E. and Hepworth, C., 2020. The influence of stomatal morphology and distribution on photosynthetic gas exchange. The Plant Journal, 101, pp. 768-779. https://doi.org/10.1111/tpj.14560

Hernández, S.M., 1990. Manual de laboratorio: Citología y citogenética. Primera edición, México: Editorial Trillas.

Hizukuri, S., Takeda, Y., Yasuda, M. and Suzuki, A., 1981. Multi-branched nature of amylose and the action of debranching enzymes. Carbohydrate Research. 94, pp. 205-213. https://doi.org/10.1016/S0008-6215(00)80718-1

Horwitz, W. 1971. Official Methods of Analysis of the Association of Official Analytical Chemists. Treceava edición. Washington, USA: AOAC International.

Instituto Mexicano de la Propiedad Industrial (IMPI), 2002. Declaración de protección a la denominación de origen sotol. Diario Oficial de la Federación. https://www.gob.mx/cms/uploads/attachment/file/494502/DO.Sotol.08.08.2002.pdf, consultado 3 de marzo, 2025.

Instituto Nacional de Estadística y Geografía (INEGI), 1988. Carta de Geología: escala 1:250 000, México. https://www.inegi.org.mx/temas/geologia/, consultado 20 de junio de 2025.

Instituto Nacional de Estadística y Geografía (INEGI), 1989. Carta de Climatología: escala 1:250 000, México. https://www.inegi.org.mx/temas/climatologia/, consultado 20 de junio de 2025.

Instituto Nacional de Estadística y Geografía (INEGI), 2000. Carta de Uso de suelo y vegetación: escala 1:250 000, México. https://www.inegi.org.mx/temas/usosuelo/, consultado 20 de junio de 2025.

Instituto Nacional de Estadística y Geografía (INEGI), 2007. Carta de Edafología: escala 1:250 000, México. https://www.inegi.org.mx/temas/edafologia/, consultado 20 de junio de 2025.

Ishfaq, M., Wang, Y., Yan, M., Wang, Z., Wu, L., Li, C. and Li, X., 2022. Physiological essence of magnesium in plants and its widespread deficiency in the farming system of China. Frontiers in Plant Science,13, pp. 802274. https://doi.org/10.3389/fpls.2022.802274

Jeandet, P., Formela-Luboi?ska, M., Labudda, M. and Morkunas, I., 2022. The Role of sugars in plant responses to stress and their regulatory function during development. International Journal of Molecular Sciences, 23, pp. 5161. https://doi.org/10.3390/ijms23095161

Johnson, R., Vishwakarma, K., Hossen, M.S., Kumar, V., Shackira, A.M., Puthur, J.T., Gholamreza, A., Mohammad, S. and Hasanuzzaman, M., 2022. Potassium in plants: Growth regulation, signaling, and environmental stress tolerance. Plant Physiology and Biochemistry. 172, pp. 56-69. https://doi.org/10.1016/j.plaphy.2022.01.001

Julius, B.T., Leach, K.A., Tran, T.M., Mertz, R.A. and Braun, D.M., 2017. Sugar transporters in plants: new insights and discoveries. Plant and Cell Physiology, 58, pp. 1442-1460. https://doi.org/10.1093/pcp/pcx090

Kardiman, R. and Raebild, A., 2018. Relationship between stomatal density, size and speed of opening in Sumatran rainforest species. Tree physiology, 38, pp. 696-705. https://doi.org/10.1093/treephys/tpx149

Kumar, V., Pandita, S., Sidhu, G.P.S., Sharma, A., Khanna, K., Kaur, P., Bali, A.S. and Setia, R., 2021. Copper bioavailability, uptake, toxicity and tolerance in plants: a comprehensive review. Chemosphere, 262, pp. 127810. https://doi.org/10.1016/j.chemosphere.2020.127810

Laughlin, D.C., Delzon, S., Clearwater, M.J., Bellingham, P.J., McGlone, M.S. and Richardson, S.J., 2020. Climatic limits of temperate rainforest tree species are explained by xylem embolism resistance among angiosperms but not among conifers. New Phytologist, 226, pp. 727-740. https://doi.org/10.1111/nph.16448

López-Salmerón, V., Cho, H., Tonn, N. and Greb, T., 2019. The phloem as a mediator of plant growth plasticity. Current Biology, 29, pp. 173-181. https://doi.org/10.1016/j.cub.2019.01.015

Madrid-Solórzano, J.M., García-Alcaraz, J.L., Macías, E.J., Cámara, E.M. and Fernández, J.B., 2021. Life cycle analysis of sotol production in México. Frontiers in Sustainable Food Systems, 5, pp. 769478. https://doi.org/10.3389/fsufs.2021.769478

Mancilla-Margalli, N.A. and López, M.G., 2006. Water-soluble carbohydrates and fructan structure patterns from Agave and Dasylirion species. Journal of Agricultural and Food Chemistry. 54, pp. 7832-7839. https://doi.org/10.1021/jf060354v

Mir, A.R., Pichtel, J. and Hayat, S., 2021. Copper: uptake, toxicity and tolerance in plants and management of Cu-contaminated soil. Biometals, 34, pp. 737–759. https://doi.org/10.1007/s10534-021-00306-z

Mitra, A., Chatterjee, S., Voronina, A.V., Walther, C. and Gupta, D.K., 2020. Lead Toxicity in Plants: A Review. In: Gupta, D., Chatterjee, S., Walther, C. eds. Lead in Plants and the Environment. Radionuclides and Heavy Metals in the Environment. Switzerland: Springer. pp. 99-116. https://doi.org/10.1007/978-3-030-21638-2_6

Negrini, A., Ferreira da Silva, R., Da Ros, C.O., Welter, P.D., Silva, A.P.D., Boeno, D. and Andreazza, R., 2024. Copper bioconcentration and bioaccumulation in different eucalyptus species and their impact on plant physiology. Journal of Plant Nutrition, 47, pp. 413-422. https://doi.org/10.1080/01904167.2023.2278653

Pandey, N., 2018. Role of plant nutrients in plant growth and physiology. In: Hasanuzzaman M, Fujita M, Oku H, Nahar K, Hawrylak-Nowak B, eds. Plant nutrients and abiotic stress tolerance. Singapore: Springer, pp. 51-93. https://doi.org/10.1007/978-981-10-9044-8_2

Pathare, V.S., Koteyeva, N. and Cousins, A.B., 2020. Increased adaxial stomatal density is associated with greater mesophyll surface area exposed to intercellular air spaces and mesophyll conductance in diverse C4 grasses. New Phytologist, 225, pp. 169-182. https://doi.org/10.1111/nph.16106

Pérez-López, E., 2013. Análisis de fertilidad de suelos en el laboratorio de Química del Recinto de Grecia, Sede de Occidente. InterSedes, 14, pp. 6-18.

Qaderi, M.M., Martel, A.B. and Dixon, S.L., 2019. Environmental factors influence plant vascular system and water regulation. Plants, 8, pp. 65. https://doi.org/10.3390/plants8030065

R Core Team, 2022. R: A language and environment for statistical computing. Viena: Austria: R Foundation. https://www.r-project.org/, consultado 10 de enero 2025.

Reyes-Valdés, M.H. and Cázares, U.F., 2022. Sotol un elixir con denominación de origen. Primera edición. México: Quintanilla ediciones.

Reyes-Valdés, M.H., Benavides-Mendoza, A., Ramírez-Rodríguez, H. and Villarreal-Quintanilla, J.A., 2013. Biología e importancia del sotol (Dasylirion spp). Parte II: Ecofisiología, usos e interrogantes. Planta, 8, pp. 16-20.

Reyes-Valdés, M.H., Palacios, R., Rivas-Martínez, E.N., Robledo-Olivo, A., Antonio-Bautista, A., Valdés-Dávila, C.M., Villarreal-Quintanilla, J.A. and Benavides-Mendoza, A., 2019. The sustainability of mexican traditional beverage sotol: ecological, historical, and technical issues. Processing and Sustainability of Beverages, 2, pp. 103-137. https://doi.org/10.1016/B978-0-12-815259-1.00004-5

Ríos-Ramírez, S.C., del Valle, J.R.E., Ortiz, G.R., Luna, J.R. and Velasco, V.V., 2021. El crecimiento de Agave angustifolia Haw. con relación a la condición nutrimental. Revista Mexicana de Ciencias Agrícolas, 12, pp. 865-873. https://doi.org/10.29312/remexca.v12i5.2638

Romero-Romero, E., Sánchez, R., Sumich, J., Añino, Y.J. and López, O.R., 2020. Variaciones morfométricas y densidad estomática en hojas de Mangifera indica bajo condiciones lumínicas contrastantes. Tecnociencia, 22, pp. 66-75. http://portal.amelica.org/ameli/jatsRepo/224/2241124005/index.html

Sakoda, K., Watanabe, T., Sukemura, S., Kobayashi, S., Nagasaki, Y., Tanaka, Y.U. and Shiraiwa, T., 2019. Genetic diversity in stomatal density among soybeans elucidated using high-throughput technique based on an algorithm for object detection. Scientific Reports, 9, pp.7610. https://doi.org/10.1038/s41598-019-44127-0

Serafín-Higuera, E.L., Antonio-Bautista, A. and Benavides-Mendoza, A., 2022. Morfología de Dasylirion cedrosanum y su correlación con imágenes en localidades del Sureste de Coahuila. Ecosistemas y Recursos Agropecuarios, 9, pp. 1-9. https://doi.org/10.19136/era.a9n1.3198

Shabbir, Z., Sardar, A., Shabbir, A., Abbas, G., Shamshad, S., Khalid, S. and Shahid, M., 2020. Copper uptake, essentiality, toxicity, detoxification and risk assessment in soil-plant environment. Chemosphere, 259, pp.127436. https://doi.org/10.1016/j.chemosphere.2020.127436

Smith, A.M. and Zeeman, S.C., 2006. Quantification of starch in plant tissues. Nature Protocols, 1, pp. 1342–1345. https://doi.org/10.1038/nprot.2006.232

Therby-Vale, R., Lacombe, B., Rhee, S.Y., Nussaume, L. and Rouached, H., 2022. Mineral nutrient signaling controls photosynthesis: focus on iron deficiency-induced chlorosis. Trends in Plant Science, 27, pp. 502-509. https://doi.org/10.1016/j.tplants.2021.11.005

Tränkner, M., Tavakol, E. and Jákli, B., 2018. Functioning of potassium and magnesium in photosynthesis, photosynthate translocation and photoprotection. Physiologia plantarum, 163, pp. 414-431. https://doi.org/10.1111/ppl.12747

Twalla, J.T., Ding, B., Cao, G., Bao, S., Li, M., Chen, X., Xiaodong, X. and Wang, J., 2021. Roles of stomata in gramineous crops growth and biomass production. Cereal Research Communications, 50, pp. 603-616. https://doi.org/10.1007/s42976-021-00216-3

Wu, S., Li, M., Zhang, C., Tan, Q., Yang, X., Sun, X., Pan, Z., Deng, X. and Hu, C., 2021. Effects of phosphorus on fruit soluble sugar and citric acid accumulations in citrus. Plant Physiology and Biochemistry, 160, pp. 73-81. https://doi.org/10.1016/j.plaphy.2021.01.015

Xu, E., Liu, Y., Gu, D., Zhan, X., Li, J., Zhou, K. and Zou, Y. 2024. Molecular mechanisms of plant responses to copper: From deficiency to excess. International Journal of Molecular Sciences, 25, pp.6993. https://doi.org/10.3390/ijms25136993

Xu, X., Du, X., Wang, F., Sha, J., Chen, Q., Tian, G., Zhu, Z., Ge, S. and Jiang, Y., 2020. Effects of potassium levels on plant growth, accumulation and distribution of carbon, and nitrate metabolism in apple dwarf rootstock seedlings. Frontiers in Plant Science, 11, pp.904. https://doi.org/10.3389/fpls.2020.00904

You, C., Wang, H., Huang, Y., Xu, P., Wu, L., Yu, F., Zhong, X., Gao, J., Zhang, L., He, H. and Ke, J., 2021. Relationship between the vascular bundle structure of panicle branches and the filling of inferior spikelets in large-panicle Japonica Rice. Frontiers in Plant Science, 12, pp. 774565. https://doi.org/10.3389/fpls.2021.774565

You, Y., Zhang, M., Yang, W., Li, C., Liu, Y., Li, C. and Wu, W., 2020. Starch phosphorylation and the in vivo regulation of starch metabolism and characteristics. International Journal of 17 Biological Macromolecules, 159, pp. 823-831. https://doi.org/10.1016/j.ijbiomac.2020.05.156

Young, M.D., Ros, G.H. and de Vries, W., 2021. Impacts of agronomic measures on crop, soil, and environmental indicators: A review and synthesis of meta-analysis. Agriculture, Ecosystems & Environment, 319, pp. 1-18. https://doi.org/10.1016/j.agee.2021.107551

Zhang, Y., Ding, C., Liu, Y., Li, S., Li, X., Xi, B. and Duan, J., 2023. Xylem anatomical and hydraulic traits vary within crown but not respond to water and nitrogen addition in Populus tomentosa. Agricultural Water Management, 278, pp. 108169. https://doi.org/10.1016/j.agwat.2023.108169

Zhao, W., Sun, Y., Kjelgren, R. and Liu, X., 2015. Response of stomatal density and bound gas exchange in leaves of maize to soil water deficit. Acta Physiologiae Plantarum, 37, pp. 1704. https://doi.org/10.1007/s11738-014-1704-8

Zhou, X.R., Zhang, N.N., Zhao, Y.M., Dai, L., Xu, D.P., Xu, G.F. and Tian, J. 2021. Distribution dynamics and roles of starch in non-photosynthetic vegetative organs of Santalum album Linn., a hemiparasitic tree. Frontiers in Plant Science, 11, pp.532537. https://doi.org/10.3389/fpls.2020.532537




URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v28i2.62435

DOI: http://dx.doi.org/10.56369/tsaes.6243

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Copyright (c) 2025 Emir Lenin Serafin-Higuera, Manuel Humberto Reyes-Valdés, Hermila Trinidad García-Osuna, América Berenice Morales-Díaz, José Ángel Villarreal-Quintanilla, Julia Medrano-Macías, Guillermina Barrientos-Rivera, Adalberto Benavides-Mendoza

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