REPORT OF PEPPER GOLDEN MOSAIC VIRUS IN NATIVE APAXTLECO CHILI PEPPER IN GUERRERO, MEXICO

Jose Francisco Díaz Nájera, Sergio Ayvar Serna, Jose Luis Arispe Vazquez, Jose Terrones Salgado, Alfredo Flores Nañez, Mateo Vargas Hernandez, Karla Vanessa De Lira Ramos, Leslie Carnero Aviles, Moises Felipe Victoriano, Juan Mayo Hernandez

Abstract


Background. The chili is a cornerstone of Mexican culture and cuisine, deeply embedded in the country's history and identity. Objective. To identify genetically and phylogenetically the virus that affects the native Apaxtleco chili pepper in Guerrero, Mexico, and to recognize symptoms of virosis in plants. Methodology. Young leaves of native Apaxtleco chili pepper plants with typical virosis symptoms were collected. The alignment of 13 sequences of DNA nucleotides was carried out, 11 of which belong to the fragment of region that codifies for the gene of “capsid protein / nuclear exportation factor BR1” of PepGMV two of these were obtained in this study, Aymedi-02 (KX641201.1) and Aymedi-01 (KX641200.1), and the nine remaining sequences were obtained from the GenBank database. Two more sequences were included for the purpose of tree rooting; one corresponds to the chlorotic leaf curl virus (AF325497.1) and another to the mosaic virus of African cassava (J02057.1), obtained from GenBank. The alignment of 13 sequences consisted of a total extension of 541 bases. To obtain descriptive information about the PepGMV sequences, only the sequences corresponding to it (11 sequences) were used, with a total of 326 conserved sites and 211 variable sites; of these, 89 are parsimony informative sites and 120 Singleton. Results. The sequence analysis showed 99% of identity with sequences of the PepGMV and the phylogenetic analysis confirmed the identification. Implications. Our results highlight a significant opportunity to enhance the control of this pathogen in this crop. Conclusion. Pepper Golden Mosaic Virus (PepGMV) was identified in Guerrero, Mexico, the symptoms presented in plants infected by this pathogen were: chlorosis, deformation, and mosaics. 

Keywords


Begomovirus; chili pepper leaf; diagnosis; nucleotide sequence.

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URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v28i2.61444

DOI: http://dx.doi.org/10.56369/tsaes.6144

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Copyright (c) 2025 Jose Francisco Díaz Nájera, Sergio Ayvar Serna, Jose Luis Arispe Vazquez, Jose Terrones Salgado, Alfredo Flores Nañez, Mateo Vargas Hernandez, Karla Vanessa De Lira Ramos, Leslie Carnero Avilés, Moises Felipe Victoriano, Juan Mayo Hernandez

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