NUTRIENT CONTENT, IN VITRO GAS PRODUCTION AND POST INCUBATION PARAMETERS OF INDIGENOUS BROWSE FODDERS IN DIFFERENT AGRO-ECOLOGICAL ZONES OF NORTHWEST ETHIOPIA

Berihun Kibret Tegegne, Bimrew Asmare Limenih, Likawent Yeheyis Engedaw, Merga Bayssa Becho

Abstract


Background: In Ethiopia indigenous browse species have the potential to serve as a sustainable supplement for poor quality feeds and enhance ruminant livestock production. Objective: To analyze the nutrient content, in vitro gas production, and post incubation parameters of indigenous browse species in northwestern Ethiopia. Methodology: Eight indigenous browse fodder trees were collected from each agroecological zone for this experiment. Leaf and pod samples of the fodder trees were collected, dried and ground for laboratory analysis. Analysis of variance was carried out for nutrient content, in vitro gas, and methane production of the samples within agroecological zones using standard analytical procedures. The statistical design used to analyze the data was Completely Randomized Design (CRD).  Results: The highest CP content was recorded from Vernonia amygdalina (223.4 g/kg DM), Dodonaea viscosa (207.7 g/kg DM), and Acacia abyssinica (216.0 g/kg DM) in the highland, midland and lowland agro-ecology respectively. In lowland area, NDF, ADF and ADL contents were highest for Albezia amara leaves and lowest for Acacia brevispica respectively. V. amygdalina, Stereospermum kunthianum and Ficus vasta had the highest gas volume at 24-hour incubation time in high, mid and lowland, respectively. The estimated organic matter digestibility, metabolizable energy and short chain fatty acid at 24 hour of incubation was the highest for V. amygdalina, S kunthianum and F. vasta in high, mid and lowland, respectively. Ficus sycomorus and Myrica salicifolia had the lowest methane production among the species in the low, mid and highland study areas, respectively. Implications: The present study provides a valuable resource for selecting suitable feed options for livestock in different agroecological zones. High CP and digestible browse species like V. amygdalina, S. kunthianum, and F. vasta could be prioritized for livestock feed. Low methane-producing species like F. sycomorus and M. salicifolia could be incorporated into diets to reduce enteric methane emissions. Conclusion: Based on their nutrient composition and in vitro gas production potential, these feed resources are of better quality to supplement grazing livestock during the seasons of critical feed shortage as the main feed resources are either limited in availability or lower in nutrient composition and digestibility in the study areas.

Keywords


Browse species; digestibility; gas production; Nutrient content; Methane production.

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References


Abraham, G., Kechero, Y. and Andualem, D., 2023. Potential of indigenous legume fodder tree and shrubs to animal feed and mitigation of methane emission in the semi-humid condition of southern Ethiopia. Legume Science, 5(4), p. e200. https://doi.org/10.1002/leg3.200

Almaz Ayenew, Adugna Tolera, Ajebu, N. and Getachew, A., 2021. Farmers’ preference and knowledge on indigenous multipurpose browse species towards their feed value in north western Ethiopia. Tropical and Subtropical Agroecosystems, 24, pp. 20. http://dx.doi.org/10.56369/tsaes.3124

Amad, A. A. and Zentek, J., 2023. The use of Moringa oleifera in ruminant feeding and its contribution to climate change mitigation. Frontiers in Animal Science, 4(1137562), pp.1–13. https://doi.org/10.3389/fanim.2023.1137562

Amanzougarene, Z. and Fondevila, M., 2020. Fitting of the in vitro gas production technique to the study of high concentrate diets. Animals, 10(10), pp. 1–13. https://doi.org/10.3390/ani10101935

AOAC., 1990. Association of official analytical chemists, 1(1). INC.Arington, USA.. https://doi.org/10.7591/cornell/9781501766534.003.0007

Aynalem, G., Simon, S. and Yisehak, K., 2020. Woody fodder species in three agro-ecological Parklands of Arba Minch Zuria Woreda, Gamo Gofa Zone, Southern Ethiopia. International Journal of Biodiversity and Conservation, 12(1), pp. 38–47. https://doi.org/10.5897/ijbc2019.1305

Badgery, W., Li, G., Simmons, A., Wood, J., Smith, R., Peck, D., Ingram, L., Durmic, Z., Cowie, A., Humphries, A., Hutton, P., Winslow, E., Vercoe, P. and Eckard, R., 2022. Reducing enteric methane of ruminants in Australian grazing systems – a review of the role for temperate legumes and herbs. Crop and Pasture Science, 74(7–8), pp. 661–679. https://doi.org/10.1071/CP22299

Balehegn, M., Duncan, A., Tolera, A., Ayantunde, A. A., Issa, S., Karimou, M., Zampaligré, N. andré, K., Gnanda, I. and Varijakshapanicker, P., 2020. Improving adoption of technologies and interventions for increasing supply of quality livestock feed in low-and middle-income countries. Global Food Security, 26, p. 100372. https://doi.org/doi.org/10.1016/j.gfs.2020.100372

Balogun, R.O., Jones, R.J. and Holmes, J.H.G., 1998. Digestibility of some tropical browse species varying in tannin content. Animal Feed Science and Technology, 76(1–2), pp. 77–88. https://doi.org/10.1016/S0377-8401(98)00210-7

Bayssa, M., Negesse, T. and Tolera, A., 2016. Leaf biomass yield, chemical composition, in vitro gas and methane production and rumen degradation characteristics of some woody plant species in Afar rangeland of North Eastern Ethiopia. Middle-East Journal of Scientific Research, 24(4), pp. 1252–1265. https://doi.org/10.5829/idosi.mejsr.2016.24.04.23362

Berhanu, Y., Olav, L., Nurfeta, A., Angassa, A. and Aune, J.B., 2019. Methane Emissions from Ruminant Livestock in Ethiopia : Promising Forage Species to Reduce CH4 Emissions. Agriculture, 9(130), pp. 1–16. https://doi.org/10.3390/agriculture9060130

Canul-Solis, J., Campos-Navarrete, M., Piñeiro-Vazquez, A., Casanova-Lugo, F., Barros Rodriguez, M., Chay Canul, A., Cardenas Medina, J. and Castillo-Sanchez, L., 2020. Mitigation of rumen methane emissions with Foliage and Pods of Tropical Trees. Animals, 10(5), pp. 1–14. https://doi.org/10.3390/ani10050843

Chebli, Y., Otmani, S. El, Chentouf, M., Hornick, J. L. and Cabaraux, J. F., 2021. Temporal variations in chemical composition, in vitro digestibility, and metabolizable energy of plant species browsed by goats in southern mediterranean forest rangeland. Animals, 11(5), pp. 1–17. https://doi.org/10.3390/ani11051441

Cheema, U. B., Sultan, J. I., Javaid, A., Mustafa, M. I. and Younas, M., 2014. Screening of fodder tree leaves by chemical composition , mineral profile , anti-nutritional factors and in sacco digestion kinetics. Scholarly Journal of Agricultural Sciences, 4(11), pp. 558–564.

Dereje, A, Mengistu, G. and Merga, B., 2021. In vitro gas production kinetics of selected multipurpose tree browses in Gelana rangelands In vitro gas production kinetics of selected multipurpose tree browses in Gelana rangelands. Livestock Research for Rural Development, 33(2), p.18. https://www.lrrd.org/lrrd33/2/a.dere3318.html

Derero, A. and Kitaw, G., 2018. Nutritive values of seven high priority indigenous fodder tree species in pastoral and agro-pastoral areas in Eastern Ethiopia. Agriculture and Food Security, 7(1), pp. 1–9. https://doi.org/10.1186/s40066-018-0216-y

Desalegn, K., Mekasha, Y. and Asefa, G., 2016. Identification and Nutritional Value Assessment of the Major Browse Species in Chilega District North Gondar. Global Veterinaria, 16, pp. 6–17. https://doi.org/10.5829/idosi.gv.2016.16.01.10217

Erdaw, M.M., 2023. Contribution, prospects and trends of livestock production in sub-Saharan Africa: a review. International Journal of Agricultural Sustainability, 21(1), pp. 2247776. https://doi.org/10.1080/14735903.2023.2247776

Food and Agriculture Organization, 2022. Versión resumida de El estado mundial de la pesca y la acuicultura 2022. Hacia la transformación azul. Rome:FAO. https://openknowledge.fao.org/server/api/core/bitstreams/05dd1625-23c4-4030-a733-247b5a48b496/content

Feyisa, T., Tolera, A., Nurfeta, A., Balehegn, M., Yigrem, S., Bedaso, M., Boneya, M. and Adesogan, A., 2022. Assessment of fodder resources in Ethiopia: Biomass production and nutritional value. Agronomy Journal, 114(1), pp. 8–25. https://doi.org/10.1002/agj2.20895

Fievez, V., Babayemi, O. J. and Demeyer, D., 2005. Estimation of direct and indirect gas production in syringes: A tool to estimate short chain fatty acid production that requires minimal laboratory facilities. Animal Feed Science and Technology, 123, pp. 197–210. https://doi.org/10.1016/j.anifeedsci.2005.05.001

Gerber, P.J., Steinfeld, H., Henderson, B., Mottet, A., Opio, C., Dijkman, J., Falcucci, A. and Tempio, G., 2013. Tackling climate change through livestock: a global assessment of emissions and mitigation opportunities. Food and Agriculture Organization, Rome, Italy. https://www.cabidigitallibrary.org/doi/full/10.5555/20133417883

Getachew, G., Makkar, H.P.S. and Becker, K., 2000. Effect of polyethylene glycol on in vitro degradability of nitrogen and microbial protein synthesis from tannin-rich browse and herbaceous legumes. British Journal of Nutrition, 84(1), pp. 73–83. https://doi.org/10.1017/s0007114500001252

Hassan Sallamab, A.S., Silva Bueno, C.da-I., Barbosa de Godoy, P.F., Nozella, E., Silber Schmidt, D.M. and Luiz Abdalla, A., 2010. Ruminal fermentation and tannins bioactivity of some browses using a semi-automated gas production technique. Tropical and Subtropical Agroecosystems, 12, pp. 1–10. https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/299

Humbelani, S.M., Hilda, K., Khuliso, E.R. and Zimbili, M., 2021. Nutrients profile of 52 browse species found in semi-arid areas of South Africa for livestock production : effect of Harvesting Site. Plants, 10(10), 2127. https://doi.org/10.3390/plants10102127

IPCC., 2006. Intergovernmental panel on climate change 2006 IPCC guidelines for national greenhouse gas inventories. Institute for Global Environmental Strategies. Washington D.C., USA.

Kellems, R.O. and Church, D.C., 1998. Livestock Feeds and Feeding (4th Edit.) Simon and Schuster. New Jersey, USA, 1998, pp.59–61.

Krishnamoorthy, U., Soller, H., Steingass, H. and Menke, K.H., 1995. Energy and protein evaluation of tropical feedstuffs for whole tract and ruminal digestion by chemical analyses and rumen inoculum studies in vitro. Animal Feed Science and Technology, 52(3–4), pp. 177–188. https://doi.org/10.1016/0377-8401(95)00734-5

Kumar, A., Ogita, S. and Yau, Y.Y., 2018. Biofuels: greenhouse gas mitigation and global warming: next generation biofuels and role of biotechnology. In Biofuels: Greenhouse Gas Mitigation and Global Warming: Next Generation Biofuels and Role of Biotechnology. University of Illinois, Urbana, USA. https://doi.org/https://doi.org/10.1007/978-81-322-3763-1

Lenné, J.M. and Thomas, D., 2006. Integrating crop livestock research and development in Sub-Saharan Africa: option, imperative or impossible? Outlook on Agriculture, 35(3), pp. 167–175. https://doi.org/https://doi.org/10.5367/000000006778536765

Makkar, H.P.S., 2003. Quantification of tannins in tree and shrub foliage: a laboratory manual. International atomic energy agency,Vienna, Austria.

Matovu, J. and Alçiçek, A., 2023. Feed resources used for small ruminant nutrition in Sub-Saharan Africa: a case study of Uganda. Tropical Animal Health and Production, 55(6), pp. 1–11. https://doi.org/10.1007/s11250-023-03781-3

Melaku, S., Aregawi, T. and Nigatu, L., 2010. Chemical composition, in vitro dry matter digestibility and in sacco degradability of selected browse species used as animal feeds under semi-arid conditions in Northern Ethiopia. Agroforestry Systems, 80(2), pp. 173–184. https://doi.org/10.1007/s10457-010-9295-x

Mengistu, A., Kebede, G., Feyissa, F. and Assefa, G., 2017. Review on Major Feed Resources in Ethiopia: Conditions, Challenges and Opportunities. Academic Research Journal of Agricultural Science and Research, 5(3), pp.176-185. https://doi.org/10.14662/ARJASR2017.013

Menke, K. and Steingass, K., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 28, pp.7-55. https://cir.nii.ac.jp/crid/1573387450798066432.bib?lang=en

Min, B.R., Solaiman, S., Waldrip, H.M., Parker, D., Todd, R.W. and Brauer, D., 2020. Dietary mitigation of enteric methane emissions from ruminants: A review of plant tannin mitigation options. Animal Nutrition, 6(3), 231–246. https://doi.org/10.1016/j.aninu.2020.05.002

Misrak K.N., 2023. Identification and nutritional value assessment of major browse species as livestock feed in Kurfa Chele district of east Hararghe zone, Ethiopia. MSc Thesis. Haramaya University, Haramaya, Ethiopia.

Mokoboki, H.K., Sebola, A.N., Ravhuhali, K.E. and Nhlane, L., 2019. Chemical composition, in vitro ruminal dry matter degradability and dry matter intake of some selected browse plants. Cogent Food and Agriculture, 5(1), pp. 1–10. https://doi.org/10.1080/23311932.2019.1587811

Mutai, A.P., Nandwa, A., Ronoh, S., Sergon. P., Oliech O., Yator, M., Meso, N.D. and Koech, K.J., 2022. Nutritive value, tannin bioassay and processing effects of Acacia brevispica, A. mellifera and A. tortilis pods as potential supplements for growing small east african goats (SEAGs) in baringo county-Kenya. African Journal of Education, Science and Technology, 7(1), pp. 5–24. http://41.89.164.27:8080/xmlui/handle/123456789/1653

Njidda, A.A. and Oloche, J., 2022. Correlation between chemical composition and in vitro Dry matter digestibility of leaves of semi-arid browses of north-east nigeria. Nigerian Agricultural Journal, 53(2), pp. 278–283. http://www.ajol.info/index.php/naj

NRC (National Research Council)., 2001. Nutrient Requirements of Dairy Cattle. National academy press, Washington, D.C. https://doi.org/10.17226/9825

Orskov, E.R. and Mcdonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science, 92(2), pp. 499–503. https://doi.org/10.1017/S0021859600063048

Palangi, V., Taghizadeh, A., Abachi, S. and Lackner, M., 2022. Strategies to Mitigate Enteric Methane Emissions in Ruminants: A Review. Sustainability, 14(20), pp. 1–15. https://doi.org/10.3390/su142013229

Patra, A.K. and Saxena, J., 2010. A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry, 71(11–12), pp. 1198–1222. https://doi.org/10.1016/j.phytochem.2010.05.010

Ravetto Enri, S., Probo, M., Renna, M., Caro, E., Lussiana, C., Battaglini, L. M., Lombardi, G. and Lonati, M., 2020. Temporal variations in leaf traits, chemical composition and in vitro true digestibility of four temperate fodder tree species. Animal Production Science, 60(5), pp. 643–658. https://doi.org/10.1071/AN18771

Sebolai, T.M., 2018. Nutritional characterization of browse plants harvested at different browsing heights in Eastern Cape province. [Ph.D. Thesis. North-West University,]. https://orcid.org/0000-0001-9115-6649

Shenkute B., Hassen A., Assafa T., Amen, N. and Ebro, A., 2012. Identification and nutritive value of potential fodder trees and shrubs in the mid Rift Valle of Ethiopia. The Journal of Animal and Plant Sciences, 22(4), pp. 1126–1132. http://hdl.handle.net/2263/56661

Sisay, A., Negesse, T. and Nurfeta, A., 2018. Short Chain Fatty Acid Production , Organic Matter Digestibility and Metabolisable Energy Content of Indigenous Browses from Ethiopian Rift Valley. IOSR Journal of Agriculture and Veterinary Science, 11(1), pp. 61–68. https://doi.org/10.9790/2380-1101016168

Terrill, T.H., Rowan, A.M., Douglas, G.B. and Barry, T.N., 1992. Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture, 58(3), pp. 321–329. https://doi.org/10.1002/jsfa.2740580306

Tilley, J.M.A. and Terry, R.A., 1963. A Two?Stage Technique for the in Vitro Digestion of Forage Crops. Grass and Forage Science, 18(2), pp. 104–111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x

Van Soest, P.J. and Robertson, J.B., 1985. Analysis of forages and fibrous foods. A laboratory manual for anumal Science. Ithaca:Cornell University.

Van Soest, P.J., Robertson, J.B. and Lewis, B.A., 1991. Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74(10), pp. 3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Yisehak, K., De Boever, J.L. and Janssens, G.P.J., 2014. The effect of supplementing leaves of four tannin-rich plant species with polyethylene glycol on digestibility and zootechnical performance of zebu bulls (Bos indicus). Journal of Animal Physiology and Animal Nutrition, 98(3), pp. 417–423. https://doi.org/10.1111/jpn.12068

Yisehak, K. and Janssens, G.P.J., 2013. Evaluation of nutritive value of leaves of tropical tanniferous trees and shrubs. Livestock Research for Rural Development, 25(2), p. 28. https://lrrd.cipav.org.co/lrrd25/2/yise25028.htm




URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v27i3.57247

DOI: http://dx.doi.org/10.56369/tsaes.5724



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