CARCASS CHARACTERISTICS AND MEAT QUALITY OF RABBITS SUPPLEMENTED WITH ZINC METHIONINE OR MARIGOLD PIGMENT AT TWO AGES AT SLAUGHTER

Pablo Antonio Garcia Martinez, Juan Edrei Sánchez Torres, Ignacio Arturo Dominguez Vara, Ernesto Morales Almaraz, Johana Paola Galeano Diaz, Luis Alberto Mejia Uribe, Benjamin Valladares Carranza, Jacinto Efrén Ramirez Bribiesca

Abstract


Background: The consumption per capita of rabbit meat in Mexico is limited (120g) due to the lack of information among consumers about the nutritional quality of rabbit meat, and at the same time, to the appearance of color, since they prefer a redder meat with more intramuscular fat. Objective: To evaluate the effect of the addition of Zinc-Methionine (Zn-Met), marigold flower pigment (FC) and two slaughter ages (MS) on carcass characteristics and meat quality in rabbits. Methodology: A total of two hundred 40-day-old rabbits (New Zealand X California, 1124±48 g initial live weight) were randomly assigned to one of 4 experimental diets and two fattening periods (68 and 82 days of age), using a completely randomized experimental design with a 2 x 2 x 2 factorial design. The control diet contained no additives, the second diet contained 200mg/kg-1 Zinc Methionine (Zn-Met), the third diet contained 80 mg/kg-1 marigold flower pigment (FC) and the fourth diet (Zn-Met*FC) contained both additives (200mg/kg-1 Zn-Met and 80 mg/kg-1 FC). The color, pH, meat-bone-to-dissectible fat ratio, water loss by cooking, shear strength and nutritional quality of rabbit meat were measured in the Longissimus thoracis et lumborum (LTL) muscle. Results: In meat color, L* values showed interactions (P < 0.05) between Zn-Met*EM and FC*EM; interaction was observed for carcass bone weight (g) (P < 0.05) between Zn-Met*FC, finding higher values (P < 0.05) in rabbits slaughtered at 82 d of age (g and %). With respect to the amount of dissectible adipose content of the carcass (g and %), there was interaction between Zn-Met*EM (P<0.05), with the highest values found in rabbits killed at 82 d (P<0.05). On the other hand, there was interaction in crude protein content (g/100g-1) (P<0.05) between Zn-Met,*FC*EM; while in moisture content and ethereal extract (g/100g-1) there was a difference (P<0.05) between animals slaughtered at different ages. MS increased the saturated fatty acid (SFA) content, while in the monounsaturated fatty acid (MUFA) content there was an interaction between Zn-Met*MS (P<0.05), with rabbits killed at 82 d of age presenting the highest values (P<0.05). Implications: The addition of Zn-Met modifies the fatty acid profile of meat in cattle and sheep, while the FC pigment modifies the color of poultry meat; however, the addition of these additives has not been studied in rabbits. Conclusion: The inclusion of Zn-Met, FC at two ages at slaughter modifies meat color and nutritional composition of rabbit meat.

Keywords


Rabbits; Marigold flower; Zinc Methionine; Productive behavior.

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References


Andrade, T.H.L.D., Pascoal, L.A.F., Martins, T.D.D., Silva, J.H.V., Silva, J.F.D., Watanabe, P.H. and Ferreira, V.C.D.S., 2018. Performance, fatty acids profile and oxidative stability of meat of rabbits fed different lipid sources. Food Science and Technology, 38(Suppl. 1), pp. 351-356. https://doi.org/10.1590/fst.20017

AOAC., 2007. Official methods of analysis. (18th ed). Maryland, USA: Association of Official Analytical Chemists.

Belew, J.B., Brooks, J.C., McKenna, D.R. and Savell, J.E., 2003. Warner–Bratzler shear evaluations of 40 bovine muscles. Meat Science, 64(4), 507-512. https://doi.org/10.1016/S0309-1740(02)00242-5

Benatmane, F., Kouba, M., Youyou, A. and Mourot, J., 2011. Effect of a linseed diet on lipogenesis, fatty acid composition and stearoyl-CoA-desaturase in rabbits. Animal, 5(12), pp. 1993-2000. https://doi.org/10.1017/S1751731111001145

Blasco, A., Ouhayoun, J. and Masoero, G., 1993. Harmonization criteria and terminology in rabbit meat research. World Rabbit Science, 1(1), pp.3-10. https://doi.org/10.4995/wrs.1993.189

Carrilho, M.C., Campo, M.M., Olleta, J.L., Beltran, J.A. and Lopez, M., 2009. Effect of diet, slaughter weight and sex on instrumental and sensory meat characteristics in rabbits. Meat Science, 82(1), pp.37-43. https://doi.org/10.1016/j.meatsci.2008.11.018

Chrastinová, ?., ?obanová, K., Chrenková, M., Polá?iková, M., Formelová, Z., Lauková, A., Ondruška, ?., Simonová-Pogány, M., Strompfová, V., Bu?ko, O., Mlyneková, Z., Mlyneková, Z., Mlynár, R. and Grešáková, ?., 2015. High dietary levels of zinc for young rabbits. Slovak Journal of Animal Science, 48(2), pp. 57-63. https://office.sjas-journal.org/index.php/sjas/article/view/186/174

CIE., 1976. Recomendations on uniform colour spaces-colour difference equations, psychometric colour terms. Supplement no.2 to CIE publication no.15 (E-1.3.1) 1978,1971/(TC-1-3). Paris France: Commision International de l´Eclairage.

Coulston, L. and Dandona, P., 1980. Insulin-like effect of zinc on adipocytes. Diabetes, 29(8), pp.665-667. https:///doi.org/10.2337/diab.29.8.665

Cruz, K.J.C., de Oliveira, A.R.S., Morais, J.B.S., Severo, J.S., Mendes, P.M.V., de Sousa Melo, S.R., Santos de Sousa, G. and do Nascimento Marreiro, D., 2018. Zinc and insulin resistance: biochemical and molecular aspects. Biological Trace Element Research, 186(2), pp. 407-412. https://doi.org/10.1007/s12011-018-1308-z

Dal Bosco, A., Mugnai, C., Roscini, V., Mattioli, S., Ruggeri, S. and Castellini, C., 2014. Effect of dietary alfalfa on the fatty acid composition and indexes of lipid metabolism of rabbit meat. Meat Science, 96(1), 606-609. https://doi.org/10.1016/j.meatsci.2013.08.027

Dalle Zotte, A., 2002. Perception of rabbit meat quality and major factors influencing the rabbit carcass and meat quality. Livestock Production Science, 75(1), pp.11-32. https://doi.org/10.1016/S0301-6226(01)00308-6

Dalle Zotte, A., Ouhayou, J., Parigi Bini R. and Xiccato, G., 1996. Effect of age, diet and sex on muscle energy metabolism and on related physicochemical traits in the rabbit. Meat Science, 43(1), pp. 15-24. https://doi.org/10.1016/0309-1740(95)00066-6

Dalle Zotte, A. and Szendr?, Z., 2011. The role of rabbit meat as functional food. Meat science, 88(3), pp.319-331. https://doi.org/10.1016/j.meatsci.2011.02.017

Deineka, V.I., Sorokopudov, V.N., Deineka, L.A. and Tret’yakov, M.Y., 2007. Flowers of marigold (Tagetes) species as a source of xanthophylls. Pharmaceutical Chemistry Journal, 41, pp.540-542. https://doi.org/10.1007/s11094-008-0007-z

Deltoro, J. and Lopez, A.M., 1985. Allometric changes during growth in rabbits The Journal of Agricultural Science, 105(2), pp.339-346. https://doi.org/10.1017/S0021859600056392

Department of Health and Social Security., 1994. Nutritional aspects of cardiovascular disease. Report on health and social subjects. London: H.M. Stationery Office.

Emken, E. A., 1994. Metabolism of dietary stearic acid relative to other fatty acids in human subjects. The American Journal of Clinical Nutrition, 60(6), pp.1023S-1028S. https://doi.org/10.1093/ajcn/60.6.1023S

Fortin, A., 1982. Carcass composition of yorkshire barrows and gilts slaughtered between 85 and 112 kg body weight. Canadian Journal of Animal Science, 62, pp. 69-76. https://doi.org/10.4141/cjas82-007

Gasperlin, L., Polak, T., Rajar, A., Skvarèa, M. and Zlender, B., 2006. Effect of genotype, age at slaughter and sex on chemical composition and sensory profile of rabbit meat. World Rabbit Science, 14(3), pp.257-166. https://doi.org/10.4995/wrs.2006.558

Greene, L.W., Lunt, D.K., Byers, F.M., Chirase, N.K., Richmond, C.E., Knutson, R.E. and Schelling, G.T., 1988. Performance and carcass quality of steers supplemented with zinc oxide or zinc methionine. Journal of Animal Science, 66(7), pp. 1818-1823. https://doi.org/10.2527/jas1988.6671818x

Hernandez, P., Aliaga, S., Pla, M. and Blasco, A., 2004. The effect of selection for growth rate and slaughter age on carcass composition and meat quality traits in rabbits. Journal of Animal Science, 82(11), pp.3138-3142. https://doi.org/10.2527/2004.82113138x

Honikel, K,O., 1998. Reference methods for the assessment of physical characteristics of meat. Meat Science, 49(4), pp.447-457. https://doi.org/10.1016/S0309-1740(98)00034-5

Huang, Q.C., Xu, Z.R., Han, X.Y. and Li, W.F., 2008. Effect of dietary betaine supplementation on lipogenic enzyme activities and fatty acid synthase mRNA expression in finishing pigs. Animal Feed Science and Technology, 140(3-4), pp.365-375. https://doi.org/10.1016/j.anifeedsci.2007.03.007

Hudon, J., 1994. Biotechnological applications of research on animal pigmentation. Biotechnology advances, 12(1), pp.49-69. https://doi.org/10.1016/0734-9750(94)90290-9

Jaramillo-Villanueva, J.L., Vargas-López, S. and Rodríguez-Guerrero, J.D., 2015. Preferencias de consumidores y disponibilidad a pagar por atributos de calidad en carne de conejo orgánico. Revista Mexicana de Ciencias Pecuarias, 6(2), pp.221-232. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242015000200007&lng=es&nrm=iso

Kessler, J., Morel, I., Dufey, P.A., Gutzwiller, A., Stern, A. and Geyer, H., 2003. Effect of organic zinc sources on performance, zinc status and carcass, meat and claw quality in fattening bulls. Livestock Production Science, 81(2-3), pp.161-171. https://doi.org/10.1016/S0301-6226(02)00262-2

Koziol, K., Maj, D. and Bieniek, J., 2015. Changes in the color and pH of rabbit meat in the aging process. Medycyna Weterynaryjna, 71(2), pp.104-108.

Lebas, F., 2013. Feeding strategy for small and medium scale rabbit units. 3rd Conference of Asian Rabbit Production Association. Bali: Indonesia.

Lin, D.S., Connor, W.E. and Spenler, C.W., 1993. Are dietary saturated, monounsaturated, and polyunsaturated fatty acids deposited to the same extent in adipose tissue of rabbits?. The American Journal of Clinical Nutrition, 58(2), pp.174-179. https://doi.org/10.1093/ajcn/58.2.174

Liu, Z.H., Lu, L., Li, S.F., Zhang, L.Y., Xi, L., Zhang, K.Y. and Luo, X.G., 2011. Effects of supplemental zinc source and level on growth performance, carcass traits, and meat quality of broilers. Poultry Science, 90(8), pp.1782-1790. https://doi.org/10.3382/ps.2010-01215

Mancini, R.A. and Hunt, M., 2005. Current research in meat color. Meat Science, 71(1), pp.100-121. https://doi.org/10.1016/j.meatsci.2005.03.003

Martínez-Alvaro, M., Agha, S., Blasco, A. and Hernández, P., 2017. Muscle lipid metabolism in two rabbit lines divergently selected for intramuscular fat. Journal of Animal Science, 95(6), pp.2576-2584. https://doi.org/10.2527/jas.2017.1371

May, J.M. and Contoreggi, C.S., 1982. The mechanism of the insulin-like effects of ionic zinc. Journal of Biological Chemistry, 257(8), pp.4362-4368. https://doi.org/10.1016/S0021-9258(18)34730-6

Miller, R.K., 2002. Factors affecting the quality of raw meat. In: J.P. Kerry, J.F. Kerry and D. Ledward, Eds., Meat Processing-Improving quality. Cambridge, England: Woodhead Publishing Co. pp. 27-63.

NOM-033-SAG/ZOO-2014. Métodos para dar muerte a los animales domesticos y silvestres. http://www.dof.gob.mx/nota_detalle.php?codigo=5405210&fecha=26/08/2015(Accessed:January2018)

Norouzi, E., Daneshyar, M., Farhoomand, P., Aliakbarlu, J. and Hamian, F., 2014. Effect of zinc acetate and magnesium sulfate dietary supplementation on broiler thigh meat colour, nutrient composition and lipid peroxidation values under continuous heat stress condition. Annals of Animal Science, 14(2), pp. 353-363. https://doi.org/10.2478/aoas-2014-0013

North, M.K., Nkhabutlane, P. and Hoffman, L.C., 2017. The effect of age on the carcass composition, portion yield and proximate composition of two rabbit genetic types in South Africa. World Rabbit Science, 25, pp.281-288. https://doi.org/10.4995/wrs.2017.6396

NRC., 1977. Nutrient Requirements of Rabbits. National Research Council.Washington, D.C., USA. National Academies Press. https://doi.org/10.17226/35

Ouhayoun, J. and Dalle Zotte, A., 1993. Muscular energy metabolism and related traits in rabbit. A review. World Rabbit Science, 1(3), pp.97-108. https://doi.org/10.4995/wrs.1993.201

Peiretti, P.G., Gai, F., Rotolo, L., Brugiapaglia, A. and Gasco, L., 2013. Effects of tomato pomace supplementation on carcass characteristics and meat quality of fattening rabbits. Meat Science, 95, pp.345-351. https://doi.org/10.1016/j.meatsci.2013.04.011

Peña, M.M., Cuevas, A.C. and González, E.A., 2004. Evaluación de tres niveles de pigmento de flor de cempasúchil (Tagetes erecta) sobre la pigmentación de la piel en pollos de engorda. Técnica Pecuaria en México, 42(1), pp.105-111. Disponible en: https://www.redalyc.org/pdf/613/61342109.pdf

Pla, M., 2008. A comparison of the carcass traits and meat quality of conventionally and organically produced rabbits. Livestock Science, 115(1), pp.1-12. https://doi.org/10.1016/j.livsci.2007.06.001

Pla, M., Guerrero, L., Guardia, D., Oliver, M. A. and Blasco, A., 1998. Carcass characteristics and meat quality of rabbit lines selected for different objectives: I. Between lines comparison. Livestock Production Science, 54(2), pp.115-123. https://doi.org/10.1016/S0301-6226(97)00179-6

Qudsieh, R. I., Smith, D. P. and Brake, J., 2018. Effect of elevated dietary inorganic zinc on live performance, carcass yield, and quality of male and female broilers. Poultry Science, 97(11), pp.1-9. https://doi.org/10.3382/ps/pey274

Rasinska, E., Czarniecka-Skubina, E. and Rutkowska, J., 2018. Fatty acid and lipid contents differentiation in cuts of rabbit meat. CyTA-Journal of Food, 16 (1), pp.807?813. https://doi.org/10.1080/19476337.2018.1488000

Rodríguez-Gaxiola, M.A., Domínguez-Vara, I.A., Barajas-Cruz, R., Mariezcurrema-Berasain, M.A., Borquez-Gastelum, J.L. and Cervantes-Pacheco, B.J., 2015. Effects of zilpaterol hydrochloride and zinc methionine on growth performance and carcass characteristics of beef bulls. Canadian Journal of Animal Science, 95(4), pp.609-615. https://doi.org/10.4141/cjas-2014-175

Rodríguez-Maya, M.A., Domínguez-Vara, I.A., Trujillo-Gutiérrez, D., Morales-Almaráz, E., Sánchez-Torres, J.E., Bórquez-Gastelum, J.L., Acosta-Dibarrat, J., Grageola-Nuñez, F. and Rodríguez-Carpena, J.G., 2019. Growth performance parameters, carcass traits, and meat quality of lambs supplemented with zinc methionine and (or) zinc oxide in feedlot system. Canadian Journal of Animal Science, 99(3), pp.585-595. https://doi.org/10.1139/cjas-2018-0153

SAS Institute Inc., 2004. SAS/STAT® 9.1 User’s Guide. Cary, NC: SAS Institute Inc. 5136 p.

Suman, S.P. and Joseph, P., 2013. Myoglobin chemistry and meat color. Annual Review of Food Science and Technology, 4, pp.79-99. https://doi.org/10.1146/annurev-food-030212-182623

Trocino, A., Filiou, E., Tazzoli, M., Birolo, M., Zuffellato, A. and Xiccato, G., 2015. Effects of floor type, stocking density, slaughter age and gender on productive and qualitative traits of rabbits reared in collective pens. Animal, 9(5), pp.855-861. https://doi.org/10.1017/S1751731114003188

Ulbricht, T.L.V. and Southgate, D.A.T., 1991. Coronary heart disease: Seven dietary factors. The Lancet, 338(8773), pp.985–992. https://doi.org/10.1016/0140-6736(91)91846-M

Wang, S., Zhang, L., Li, J., Cong, J., Gao, F. and Zhou, G., 2017. Effects of dietary marigold extract supplementation on growth performance, pigmentation, antioxidant capacity and meat quality in broiler chickens. Asian-Australasian Journal of Animal Sciences, 30(1), pp.71-77. https://doi.org/10.5713/ajas.16.0075

Wen, M., Wu, B., Zhao, H., Liu, G., Chen, X., Tian, G., Cai, J. and Jia, G., 2019. Effects of dietary zinc on carcass traits, meat quality, antioxidant status, and tissue zinc accumulation of pekin ducks. Biological Trace Element Research, 190(1), pp.187-196. https://doi.org/10.1007/s12011-018-1534-4

Wood, J.D., Enser, M., Fisher, A.V., Nute, G.R., Sheard, P.R., Richardson, R.I., Hughes, S.I. and Whittington, F.M., 2008. Fat deposition, fatty acid composition and meat quality: A review. Meat Science, 78(4), pp. 343-358. https://doi.org/10.1016/j.meatsci.2007.07.019

Yan, J.Y., Zhang, G.W., Zhang, C., Tang, L. and Kuang, S.Y., 2017.Effect of dietary organic zinc sources on growth performance, incidence of diarrhoea, serum and tissue zinc concentrations, and intestinal morphology in growing rabbits. World Rabbit Science, 25, pp.43-49. https://doi.org/10.4995/wrs.2017.5770

Young, O.A., Priolo, A., Simmons, N.J. and West, J., 1999. Effects of rigor attainment temperature on meat blooming and colour on display. Meat Science, 52(1), pp. 47-56. https://doi.org/10.1016/S0309-1740(98)00147-8

Zhang, H.B., Wang, M.S., Wang, Z.S., Zhou, A.M., Zhang, X.M., Dong, X.W. and Peng, Q.H., 2014. Supplementation dietary zinc levels on growth performance, carcass traits, and intramuscular fat deposition in weaned piglets. Biological Trace Element Research, 161(1), pp.69-77. https://doi.org/10.1007/s12011-014-0078-5

Zomeño, C., Blasco, A. and Hernández, P., 2013. Divergent selection for intramuscular fat content in rabbits. II. Correlated responses on carcass and meat quality traits. Journal of Animal Science, 91(9), pp. 4532-4539. https://doi.org/10.2527/jas2013-6417




URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v28i1.54418

DOI: http://dx.doi.org/10.56369/tsaes.5441

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Copyright (c) 2025 Juan Edrei Sánchez Torres, Pablo Antonio Garcia Martinez, Ignacio Arturo Dominguez Vara, Ernesto Morales Almaraz, Johana Paola Galeano Diaz, Luis Alberto Mejia Uribe, Benjamin Valladares Carranza, Jacinto Efrén Ramirez Bribiesca

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