USE OF RITA® TEMPORARY IMMERSION SYSTEM TO OBTAIN MICROTUBERS OF SEVERAL MASHUA (Tropaeolum tuberosum Ruiz & Pavón) MORPHOTYPES

Gilmar Peña, Roxana Carhuaz, Juan Davalos, Vidalina Andía Ayme

Abstract


Background. The Temporary Immersion System (TIS) is an effective strategy to obtain quality mashua microtubers through the in vitro propagation of seedlings in a small space and in a short period of time. Objective. Obtain microtubers of 27 mashua morphotypes (Tropaeolum tuberosum) using the TIS-RITA®. Methodology. The seedlings were previously propagated in MS (Murashige & Skoog) solid medium, supplemented with 3% sucrose, at 5.6 pH and 0.7% agar. After fifteen days of cultivation in this medium, the seedlings were transferred to a MS liquid. For the tuberization process, seedlings with an average size of 10 cm were transferred to TIS-RITA® systems for ten weeks, under conditions of complete darkness, at 19 ± 2 ° C, an immersion frequency of 2 minutes every 3 hours in a MS liquid medium supplemented with 8% sucrose and 2 mg.L-1 benzylamino purine (BAP). Results. The best results of microtubers were obtained with different morphotypes, which are respect to i/size, MAC 06A (1.9 cm) and MAC 056 (1.8 cm); ii / fresh weight, MAC 06A (0.2g) and MAC 092 (0.18 g); iii / quantity, MAC 08A (51 units) and MAC 048 (56 units). Implications. TIS-RITA® can be used to obtain quality and virus-free seeds of a wide variety of commercially important crops. Conclusion. The SIT-RITA® based on the intermittent contact of the explants cultivating medium, is an efficient system to obtain mashua microtubers.

Keywords


Microtubers; temporary immersion system SIT; mashua; Tropaeolum tuberosum; in vitro-tuberization

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URN: http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v23i3.2960



Copyright (c) 2020 Gilmar Peña, Roxana Carhuaz, Juan Davalos

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