In vitro PROPAGATION OF Coryphantha retusa (Britton & Rose) AN ENDEMIC AND ENDANGERED CACTUS

Abstract

The objective of this study was to regenerate complete plants of Coryphanta retusa (Britton & Rose) by direct organogenesis via. The tratments consisted on Murashige and Skoog (MS) basal medium supplemented with 0.0, 1.0, 2.0, 3.0 mg/L of 6-(benzylaminopurine) (6-BAP), 0.0, 0.5, 1.0 mg/L (naftalenacetic) naphthalenacetic (NAA) acid. After 60 days the axillary bud proliferation were evaluated. Response variables were germination, shoots regeneration. Results were analyzed by analysis of variance (P<0.05) and means were compared by Duncan’s multiple range test. A highest axillary bud proliferation were observed on a medium containing 2 mg/L 6-BAP. The plantlets were rooted on MS medium without (plant) growth regulators. The 94 % of regenerated plants showed good overall growth and were phenotipycally similar to the mother plants.