IN VITRO ANTHELMINTIC EFFECT OF METHANOLIC LEAF EXTRACT OF Gliricidia sepium AGAINST GASTROINTESTINALE NEMATODES OF SHEEP

Abstract

The use of local resources for food and health care of animals is a highly profitable and sustainable strategy. Among these resources are native trees and shrubs which in addition to providing good quality nutrients, produce secondary metabolites with anthelmintic (AH) effect. Therefore the aim of this study was to evaluate the in vitro AH effect of Gliricidia sepium leaves methanol extract (GSME), through the egg hatch inhibition assay (EHA). Three concentrations of the extracts were tested: 125, 250 and 500 μg/mL.  Also a negative control (distilled water) and a positive control (levamisole 2 mg/mL) were included. The GSME showed significant differences P<0.05 when compared with the positive control. The GSME also showed a dose-dependent response in inhibition of eggs hatching. Effectiveness percentages found were: 27.7%, 46.2%, 49.7% of inhibition at 125, 250, and 500 μg/mL respectively. The average dose (ED50) obtained through probit analysis was 394.96 μg/mL. These results suggest that the ME of leaves of G. sepium has anthelmintic activity against eggs of gastrointestinal nematodes.