In vitro EVALUATION OF THE NEMATICIDAL ACTIVITY OF LIMONENE, ALLYL ISOTHIOCYANATE, EUCALYPTOL, β-CITRONELLOL AND AZADIRACHTIN ON Meloidogyne incognita (Nematoda, Meloidogynidae)

Abstract

Meloidogyne incognita is one of the main biological agents that affects tomato production, alternatives to chemical pesticides are currently being evaluated, looking for compounds for nematode control. The objective of the study was to evaluate the nematicidal activity of limonene, allyl isothiocyanate, eucalyptol (2000, 1000, 500, 250, 100 and 0 ppm), β-citronellol (200, 100, 50, 25, 10, 0 ppm) and azadirachtin (12000, 10000, 8000, 6000, 4000, 0 ppm), on J2 of M. incognita. Samples were carried out in greenhouses in the State of Morelos, where soil and root samples were taken, to obtain the nematodes. The bioassays were carried out following the Cristóbal - Alejo methodology, in a controlled environment, the biological activity of five compounds was evaluated, under six treatments with five repetitions each; and as an experimental unit a Petri dish with 30 juveniles (J2) of M. incognita was used and 3 mL of each concentration was added to see the mortality response, the evaluations were carried out at 24 hours considering dead individuals they responded to the stimulation of the entomological pin, the correction of mortality was made by Abbott's formula and the mean lethal concentration was obtained using SAS 9.0. The mortality results were between 12 and 87.9%, however; β-citronellol was the one that presented the highest mortality rates and lowest LC50, so it can be considered as an alternative for nematode control.