PHENOLS AND FLAVONOIDS CONCENTRATION AND FUNGISTATIC ACTIVITY OF WOOD AND BARK OF FIVE COMMON TROPICAL SPECIES

Abstract

This research determine the total phenol and flavonoids content as well as the fungistatic activity of hot water wood sawdust and bark extracts on Coniophora puteana and Trametes versicolor. Extracts tested were taken from Condalia hookeri M.C. Johnst., Ebenopsis ebano (Berl.) Britton et Rose, Helietta parvifolia (Gray) Benth, Leucaena leucocephala (Lam.) de Wit, and Prosopis laevigata (Humb. et Bonpl. ex Willd). The extraction was developed with soxhlet aparats, phenol concentration was determined with Folin-Ciocalteu method and flavonoide was determined by Heimler procedure. Phenol concentration ranged between 50±11 to 827±23 mg g-1 and flavonoids content between 15±2 to 708±30 mg g-1. All extracts tested inhibited growth of the fungal species. The highest inhibition effect (88%±1) occurred on C. puteana with L. leucocephala wood sawdust extracts at 2 mg ml-1, this species also reduced the growth on T. versicolor by 75%±12 when used at 10 mg ml-1, wood sawdust extracts of H. parvifolia and C. hookerii at same concentration reduced the growth of T. versicolor in 43%±3 and 40%±4 respectively. Inhibition of bark extracts of E. ebano was 84±5 and 80±7 % for H. parvifolia. A negative relationship between growing inhibitory activity and the content of total phenolics in the extracts was obtained.