O. John Makinde, Aminu Maidala, I. Amaza Bagudu, Akeem B. Sikiru, Okunale S. Adewale, Eniwaiye A. Adetutu, Emmanuel Opoola, O. Solomon Ajide, A. Johnson Ajibade


Background. High cost of feed ingredients and increased demand for broiler chicken in many African countries has necessitated the search for alternative feed sources. Objective. To evaluate the effects of Hyphaene thebaica seed meal (HTSM) as substitute for maize on growth and blood parameters of broiler chickens. Methodology. Five experimental diets were formulated for the trial. Diet 1 (0% HTSM) served as the control while diets 2, 3, 4 and 5 contained 5, 10, 15 and 20% HTSM respectively replacing dietary maize in the diets of birds. A total of 225 day-old broiler chicks were randomly allotted into five treatment groups with three replicates of fifteen birds each. Each group was assigned to the five experimental diets in completely randomized design (CRD). Feed and water were supplied ad libitum for all treatment groups during the trial. Result. Differences (P<0.05) were observed in the growth parameters measured except average daily feed intake (P>0.05). Feed conversion ratio and average daily weight gain of birds fed 0, 5 and 10% HTSM diets were better (P<0.05) compared to those fed 15 and 20 % HTSM diets. Differences (P<0.05) were also observed in the blood parameters measured except the packed cell volume (29.08- 31.89%) and mean corpuscular haemoglobin (14.69-15.67pg). The white blood cell (11.45-18.14 x 109/l), red blood cell (4.71-6.99 x 1012/l), haemoglobin (6.92-10.05g/dl) and aspartate transaminase (72.95-90.16 IU/l), alanine aminotransaminase (62.84-79.50 IUu/l) and alkaline phosphatase (100.26-108.77 IU/l) increased (P<0.05) as the dietary levels of HTSM increased across the treatments. Carcass and organ weights of birds fed 0, 5 and 10% HTSM diets were better (P<0.05) than those fed 15 and 20% HTSM diets. Implication. Adequate processing of the HTSM is needed to reduce the anti-nutritional factors to a tolerable level before being used in broiler diet. Conclusion. Broiler chickens can tolerate up to 10% raw HTSM in their diets without adverse effect on growth performance. 


broiler; performance; haematology; serum; hyphaene thebaica seed meal

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Abe, H.A., Kimura, T. and Yamuchi, K., 1996. Effect of collagen on toughness of meat from spent laying hens. Journal of Food Science and Technology, 43, pp. 831-834.

Abu Hafsa, S.H., Hassan, A.A., Elghandour, M.M., Barbabosa-Pliego, A., Mellado, M. and Salem, A.Z., 2022. Dietary Anti-nutritional Factors and Their Roles in Livestock Nutrition. Sustainable Agriculture Reviews, 57, pp. 131-174. Springer, Cham.

Adeyemo, I.A. and Sani, A., 2013. Haematological Parameters and Serum Biochemical Indices of Broiler Chickens fed Aspergillus niger Hydrolyzed Cassava Peel Meal Based Diet. International Journal of Research and Reviews in Applied Sciences, 15(3), pp. 410-415.

Annongu, A.A., Joseph, K.L., Adeyina, A.O., Sola-Ojo, F.E., Edoh, J.H. and Ajide, S.O. 2017. Utilization of African Star Apple (Chrysophyllum albidum) kernel meal in broiler diets. Journal of Agricultural Sciences, 62(20), pp. 143-154.

AOAC., 2006. Association of Official Analytical Chemists Official Method of Analysis of the AOAC (W.Horwitz Editor) 18th Edition. Washighton D.C, AOAC.

Arslam, M., Ozcan, E. M., Matur, E., Cotelioghi, U. and Ergui, E., 2001. The effects of vitamin E on some blood parameters in broilers. Turkish Journal of Veterinary and Animal Sciences, 25, pp. 711 – 716.

Aziz, F., Jorgenson, M. and Garg, N. 2022. Secondary oxalate nephropathy and kidney transplantation. Current Opinion in Organ Transplantation, 28(1), pp. 15-21.

Bello, M.O., Farade, O.S., Adewusi, S.R.A. and Olawore, N.O., 2008. Studies on chemical compositions and anti nutrients of some lesser known Nigerian fruits. African Journal of Biotechnology, 7, pp. 3972-3979.

Betty, H., Coupar, M.I. and Ken, N., 2006. Antioxidant activity of hot water extract from the fruit of the Duom palm “Hyphaene thebaica”. Food Chemistry, 98, pp. 317–328.

Burkill, H.M., 1997. The useful plants of West Tropical Africa, 2nd (Edn), Royal Botanical garden, Kew, 4, pp. 371-373.

Cheeke, P.R., 1996. Biological effects of feed and forage saponins and their impacts on animal production. In: Saponins used in Food and Agriculture. G. Waller and K. Yamasaki, eds. Plenum Press, New York, USA. pp. 377-385.

Cook, J.A., VanderJagt, D.J., Pastuszyn, A., Mounkaila, G., Glew, R.S., Millison, M. and Glew, H.R., 2000. Nutritional and chemical composition of 13 wild plant foods of Niger. Journal of Food Components Analysis, 13, pp. 83–92.

Eissa, H.A., Ramadan M.T. and Ali, H.S., 2008. Effect of natural extracts from the fruits of doum palm, carob and licorice on the quality and safety of apple slices. Journal of Agricultural Science, Mansoura University, 33, pp. 6609–6623.

Emenalum, O.O., Esonu, B.O, Etuk, E.B. and Anaba, C., 2009. Effect of Mucuna pruriens (Velvet Beans) leaf meal on performance and blood composition of finisher broiler chickens. Nigerian Journal of Animal Production, 36(1), pp. 52-60.

Ermer, T., Nazzal, L., Tio, M.C., Waikar, S., Aronson, P.S. and Knauf, F., 2022. Oxalate homeostasis. Nature Reviews Nephrology, pp. 1-16.

Ibe, E.A., Kudu, Y.S., Ayanwale, B.A. and Malik, A.A., 2021. Effect of processed doum palm (Hyphaene thebaica) pulp meal on growth performance, nutrient digestibility, carcass characteristics and haematological indices of broilers. Journal of Animal Science and Veterinary Medicine, 6(5), pp. 182-187.

Islam, F., Saeed, F., Afzaal, M., Hussain, M., Al Jbawi, E., Armghan Khalid, M. and Asif Khan, M., 2022. Nutritional and functional properties of Hyphaene thebaica L. flour: a critical treatise and review. International Journal of Food Properties, 25(1), pp. 1234-1245.

Jain, N.C., 1993. Essentials of Veterinary Haematology, 4th ed. Lea and Febiger, Philadelphia, U. S. A.

Jiya, E.Z., Ijaiya, T.A., Alabi, O. J., Makinde, O.J. and Saidu, S., 2018. Response of Growing Rabbits (Oryctolagus cuniculus) fed diets containing raw Tallow (Detarium microcarpum) Seed Meal. Journal of Agricultural Sciences. 63(2), pp. 171-183.

Guyomard, H., Manceron, S. and Peyraud, J., 2013. Trade in feed grains, animals, and animal products: Current trends, future prospects, and main issues. Animal Frontiers, 3(1), pp. 14-18.

Hosseintabar-Ghasemabad, B., Janmohammadi, H., Hosseinkhani, A., Amirdahri, S., Baghban-Kanani, P., Gorlov, I.F., Slozhenkina, M.I., Mosolov, A.A., Ramirez, L.S. and Seidavi, A., 2022. Effects of Using Processed Amaranth Grain with and without Enzyme on Performance, Egg Quality, Antioxidant Status and Lipid Profile of Blood and Yolk Cholesterol in Laying Hens. Animals, 12(22), pp. 3123.

Makinde, O.J., Aremu, A., Alabi, O.J., Jiya, E.Z. and Ibe, E.A., 2017. Roasted African Star Apple (Chrysophyllum albidum) Kernel Meal improves growth performance of Growing Rabbits. Tropical and Subtropical Agroecosystems, 20 (3), pp. 457–464.

Makinde, O.J., Maidala, A., Sikiru, A.B., Adejumo, I.O., Badmus, K.A, Mohammed I.C., Dunya, A.M. and Abdullahi, A.M., 2018. Performance and Cost-Benefit Analysis of Broiler Chickens Fed Doum Palm (Hyphaene thebaica) Seed Meal Based Diet. 52nd Annual Conference of Agricultural Society of Nigeria. 22-26 October 2018. ARCN, Abuja. Pp. 13-16.

Makinde, O.J., Ajibade, A.J., Opoola, E., Sikiru, A.B. and Okunade, S.A., 2021. Potential of Entada africana guill. & perr. Seed meal as feed ingredient in the diet of broiler chickens. Tropical and Subtropical Agroecosystems, 24, pp. #79.

Makinde, O.J., Musa, A.R., Alao, J.S., Escribano, F., Kohn, R., Iglesias, E., Aminu, M., Maina, A., Mohammad, C., Charles, H, and Okunade, S.A., 2022. Cattail (Typha domingensis) silage improves feed intake, blood profile, economics of production and growth performance of beef cattle. Tropical Animal Health and Production, 54, pp. 48.

Makinde, O.J., Ayo, A., Alabi, O.J., Jiya, E.Z., Sikiru, A.B., Opoola, E., and Okunade, S.A., 2023. Growth performance and haemato-biochemical indices of growing rabbits fed African Star Apple (Chrysophyllum albidum) kernel meal. Archivos de Zootecnia.72 (279), pp. 194-201.

Martens, S.D., Tiemann, T.T., Bindelle, J., Peters, M. and Lascano, C.E., 2013. Alternative plant protein sources for pigs and chickens in the tropics–nutritional value and constraints: A review. Journal of Agriculture and Rural Development, 113(2), pp. 101-123.

Mitruka, B.M. and Rawnsley, H.M., 1977. Clinical, Biochemical and Haematological Reference Value in Normal Experimental Animals. Mason Publishing Company, New York. Pp. 35-50.

National Research Council., 1994. Nutrient Requirement of Poultry. 9th Revised Edition. National Academy Press, Washinton D.C. pp. 19-34.

Nwosu, F.O., Dosumu, O.O. and Okocha, J.O.C., 2008. The potential of Terminalia catappa (Almond) and Hyphaene thebaica (Doum palm) fruits as raw materials for livestock feed. African Journal of Biotechnology, 7, pp. 4576–4580.

Obun, C.O. and Adeyemi, O.A., 2012. Effects of raw and toasted Daniellia oliveri Rolfe seed meal as replacement for groundnut meal on the performance of broiler chickens. Revista Científica UDO Agrícola, 12 (4), pp. 947-954.

Ogbuewu, I.P., 2008. Physiological responses of rabbits fed graded levels of Neem (Azadiractha indica A Juss) leaf meal. M.Sc. Thesis, Department of Animal science and Technology, Federal University of Technology, Owerri, pp. 254.

Olorede, B.S.R., Onifade, A.A., Okpara, O.A. and Babatunde, G.M., 1996. Growth, nutrient retention, haematology and serum biochemistry of broiler chickens fed shea butter cake and palm kernel cake in humid tropics. Journal of Applied Animal Research, 4, pp. 173-180.

Oloruntola, O.D., Agbede, J.O., Onibi, G.E. and Igbasan, F.A., 2016. Replacement value of rumen liquor fermented cassava peels for maize in growing rabbit diet. Archivos de Zootecnia, 65 (249), pp. 89-97.

Oluyemi, F.O., Oyewale, J.O. and Omolewa, O.F., 2002. Plasma chemistry value in young and adult Nigerian duck (Anas plotymuchus). Israel Veterinary Medicine Association, 157(4).

Pauzenga, U., 1985. Feeding Parent Stock. Zootech International. Pp. 22-25.

SAS., 2015. User’s guide: Statistics. Version 12.0. SAS Institute, Inc. Cary. NC, USA.

USDA., 2012. Livestock and poultry: World markets and trade. USDA Foreign Agricultural Service, Washington, DC.



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