HISTOPATHOLOGIC EFFECT OF Brucella abortus ON CHICKEN EMBRYOS INOCULATED WITH MILK FROM SEROPOSITIVE COWS

Abstract

The histopathologic effect caused by B. abortus on chicken embryos (CE) inoculated with milk from seropositive cows was determined. One hundred 7- to 9-days-old CE were used and divided into three groups: I) 80 CE divided into eight subgroups of 10 CE each, that were inoculated with milk cream from eigth cows seropositive to B. abortus; Group II) 10 CE inoculated with a field strain of B. abortus (positive control group); and Group III) 10 CE inoculated with ultra-pasteurized milk (negative control group). Bacteria with characteristics resembling Brucella spp. were isolated in at least 1 of each 10 CE in Group I, and in all the CE in Group II. The CE worked as a model to achieve infection and induction of damages into the cytoarchitecture of the liver and chorioallantoic membrane, this determined by the observation of inflammation and necrosis in their tissues, both in Group I and Group II. In conclusion, B. abortus caused morphological changes in the liver and chorioallantoic membrane of CE.