OVEREXPRESSION OF WUSCHEL IMPROVES THE INDUCTION OF EMBRYOGENIC CALLUS IN SCALPS OF MUSA ACUMINATA L. AAA, CV. “GRAND NAIN”
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| 1. | Title | Title of document | OVEREXPRESSION OF WUSCHEL IMPROVES THE INDUCTION OF EMBRYOGENIC CALLUS IN SCALPS OF MUSA ACUMINATA L. AAA, CV. “GRAND NAIN” |
| 2. | Creator | Author's name, affiliation, country | Ana Ly Arroyo Herrera; Laboratorio de Farmacología Facultad de Química, Universidad Autónoma de Yucatán, Calle 43 s/n x 96 Paseo de las Fuentes y 40 Col. Inalámbrica. C.P. 97069 Mérida, Yucatán, México.; Mexico |
| 2. | Creator | Author's name, affiliation, country | Angela Kú González; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43No. 130 Colonia Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México. |
| 2. | Creator | Author's name, affiliation, country | Rosa María Escobedo Gracia-Medrano; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43No. 130 Colonia Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México.; Mexico |
| 2. | Creator | Author's name, affiliation, country | Suemy Terezita Echeverria Echeverria; Centro Bachillerato Tecnológico Agropecuario (CBTA) 13, 3Centro Bachillerato Tecnológico Agropecuario (CBTA) 13, Ex-Hacienda Xmatkuil A.P. 970 C.P. 97139, X’Matkuil, Yucatán, México.; Mexico |
| 2. | Creator | Author's name, affiliation, country | Miguel Angel Herrera Alamillo; Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43No. 130 Colonia Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México.; Mexico |
| 2. | Creator | Author's name, affiliation, country | Luis Joel Figueroa Yañez; Unidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Av. Normalistas 800 Colinas de La Normal 44270 Guadalajara, Jalisco, México.; Mexico |
| 2. | Creator | Author's name, affiliation, country | Enrique Castaño; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43No. 130 Colonia Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México.; Mexico |
| 2. | Creator | Author's name, affiliation, country | Luis Carlos Rodríguez Zapata; Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43No. 130 Colonia Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México; Mexico |
| 3. | Subject | Discipline(s) | |
| 3. | Subject | Keyword(s) | A. tumefaciens; Musa acuminata; Estradiol derivatives; Genetic transformation; Homeobox; Somatic embryogenesis; WUSCHEL. |
| 4. | Description | Abstract | Background: During the last few years the home box transcription factor WUSCHEL (WUS) has been shown to cause dedifferentiation when expressed on somatic cells followed by a production of new stem cells that can lead to somatic embryogenesis or organogenesis. WUS has been shown to promote the transition from a vegetative to an embryogenic state when overexpressed. Objective/Hypothesis. The genetic in vitro transformation of meristematic tissue of Musa acuminata L. AAA, cv. “Grand Nain” was carried out using a heterologous gene WUSCHEL from Arabidopsis thaliana, via vacuum infiltration with Agrobacterium tumefaciens, to establish and ascertain if its expression modifies the progression of the explants to the embryogenesis process and or reduces the time needed for in vitro embryogenic induction phase. Methodology: Explants of proliferating shoot meristems, named “scalps”, of Musa acuminata L. AAA, cv. “Grand Nain” was transformed with WUS gene under the control of promoter GAL4, inducible by 17b-estradiol. The effect of steroid 17b-estradiol (b-Est) and homobrassinolide (HomoBra) on the in vitro somatic embryogenesis induction phase of non-transformed banana scalps was investigated. Results: The successful transformation of the explants was confirmed by PCR, for the transferred neomycin phosphotransferase II (NPTII) and the WUS gene. In addition, the expression of the red fluorescent protein (RFP) for the corresponding transferred reporter gene was verified by fluorescence microscopy in proliferated transformed tissues. Besides, the transformed tissue response to the induction of embryogenesis with either b-Est and/or HomoBra, inducers of the transgene were investigated. Implications: The improvement of the process of somatic embryogenesis in this way, generates a more effective and productive study model in a short time. Conclusions: WUS can promote the meristematic tissue-to-embryonic transition, and eventually somatic embryo formation, suggesting that the homeodomain protein can play a critical role during embryogenesis. |
| 5. | Publisher | Organizing agency, location | Universidad Autónoma de Yucatan |
| 6. | Contributor | Sponsor(s) | Consejo Nacional de Ciencia y Tecnología (CONACYT) |
| 7. | Date | (YYYY-MM-DD) | 2023-11-07 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | |
| 9. | Format | File format | |
| 10. | Identifier | Uniform Resource Identifier | https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/4751 |
| 10. | Identifier | Uniform Resource Name (URN) | http://www.revista.ccba.uady.mx/urn:ISSN:1870-0462-tsaes.v27i1.47516 |
| 10. | Identifier | Digital Object Identifier (DOI) | http://dx.doi.org/10.56369/tsaes.4751 |
| 11. | Source | Title; vol., no. (year) | Tropical and Subtropical Agroecosystems; Vol 27, No 1 (2024): (January - April) |
| 12. | Language | English=en | en |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
Copyright (c) 2023 Miguel Angel Herrera Alamillo, ANA LY ARROYO HERRERA, ANGELA KÚ GONZÁLEZ, ROSA ESCOBEDO.GRACIA.MEDRANO, SUEMY TEREZITA ECHEVERRIA ECHEVERRIA, LUIS FIGUEROA YAÑEZ, ENRIQUE CASTAÑO, LUIS CARLOS RODRÍGUEZ ZAPATA This work is licensed under a Creative Commons Attribution 4.0 International License. |